Assessing the in vivo ameliorative effects of Lactobacillus acidophilus KLDS1.0901 for induced non-alcoholic fatty liver disease treatment

Yanbo Wang; Zengbo Wang; Yang Wan; Furong Jin; Xiaodan Shi; Zhishuang Xing; Bo Tian; Bailiang Li

doi:10.3389/fnut.2023.1147423

Assessing the in vivo ameliorative effects of Lactobacillus acidophilus KLDS1.0901 for induced non-alcoholic fatty liver disease treatment

Front Nutr. 2023 Mar 20:10:1147423. doi: 10.3389/fnut.2023.1147423. eCollection 2023.

Authors

Yanbo Wang^{1

2}, Zengbo Wang^{1

2}, Yang Wan^{1

2}, Furong Jin^{1

2}, Xiaodan Shi^{1

2}, Zhishuang Xing^{1

2}, Bo Tian^{1

2}, Bailiang Li^{1

2}

Affiliations

¹ College of Food, Northeast Agricultural University, Harbin, China.
² Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin, China.

Abstract

Reputed as a significant metabolic disorder, non-alcoholic fatty liver disease (NAFLD) is characterized by high-fat deposits in the liver and causes substantial economic challenges to any country's workforce. Previous studies have indicated that some lactic acid bacteria may effectively prevent or treat NAFLD. Overall, L. acidophilus KLDS1.0901 protected against HFD-induced NAFLD by improving liver characteristics and modulating microbiota composition, and thus could be a candidate for improving NAFLD. This study aimed to assess the protective effects of L. acidophilus KLDS1.0901 on a high-fat diet(HFD)-induced NAFLD. First, hepatic lipid profile and histological alterations were determined to study whether L. acidophilus KLDS1.0901 could ameliorate NAFLD. Then, the intestinal permeability and gut barrier were explored. Finally, gut microbiota was analyzed to elucidate the mechanism from the insights of the gut-liver axis. The results showed that Lactobacillus KLDS1.0901 administration significantly decreased body weight, Lee's index body, fat rate, and liver index. L. acidophilus KLDS1.0901 administration significantly improved lipid profiles by decreasing the hepatic levels of total cholesterol (TC), triglyceride (TG), and low-density lipoprotein cholesterol (LDL-C) and by increasing the high-density lipoprotein cholesterol (HDL-C) levels. A conspicuous decrease of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum was observed after L. acidophilus KLDS1.0901 administration. Meanwhile, the H&E and Oil Red O-stained staining showed that L. acidophilus KLDS1.0901 significantly reduced liver lipid accumulation of HFD-fed mice by decreasing the NAS score and lipid area per total area. Our results showed that L. acidophilus KLDS1.0901 administration decreased the interleukin-6 (IL-6), interleukin-1β (IL-1β), and tumor necrosis factor-alpha (TNF-α) concentrations accompanied by the increase of interleukin-10 (IL-10). L. acidophilus KLDS1.0901 administration could improve the intestinal barrier function by upregulating the mRNA levels of occludin, claudin-1, ZO-1, and Muc-2, which were coupled to the decreases of the concentration of LPS and D-lactic acid. Notably, L. acidophilus KLDS1.0901 administration modulated the gut microbiota to a near-normal pattern. Hence, our results suggested that L. acidophilus KLDS1.0901 can be used as a candidate to ameliorate NAFLD.

Keywords: Lactobacillus acidophilus; gut microbiota; high-fat diet; lipid accumulation; non-alcoholic fatty liver disease.

Grants and funding

The present research study was financially supported by the National Natural Science Foundation of China (32101919), the Young Elite Scientist Sponsorship Program by CAST (YESS20200271), and the China Postdoctoral Science Foundation (2022M721071).