Analysis of starch dissolved in ionic liquid by glass nanopore at single molecular level

Wanyi Xie; Shixuan He; Shaoxi Fang; Bohua Yin; Rong Tian; Yunjiao Wang; Deqiang Wang

doi:10.1016/j.ijbiomac.2023.124271

Analysis of starch dissolved in ionic liquid by glass nanopore at single molecular level

Int J Biol Macromol. 2023 Jun 1:239:124271. doi: 10.1016/j.ijbiomac.2023.124271. Epub 2023 Apr 3.

Authors

Wanyi Xie¹, Shixuan He², Shaoxi Fang¹, Bohua Yin³, Rong Tian¹, Yunjiao Wang¹, Deqiang Wang⁴

Affiliations

¹ Chongqing Key Laboratory of Multi-scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China; Chongqing School, University of Chinese Academy of Science, Chongqing 400714, China.
² Chongqing Key Laboratory of Multi-scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China; Chongqing School, University of Chinese Academy of Science, Chongqing 400714, China. Electronic address: heshixuan@cigit.ac.cn.
³ Chongqing Key Laboratory of Multi-scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China; Changchun University of Science and Technology, Changchun 130022, China.
⁴ Chongqing Key Laboratory of Multi-scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China; Chongqing School, University of Chinese Academy of Science, Chongqing 400714, China. Electronic address: dqwang@cigit.ac.cn.

PMID: 37019197
DOI: 10.1016/j.ijbiomac.2023.124271

Abstract

In this paper, the glass nanopore technology was proposed to detect a single molecule of starch dissolved in ionic liquid [1-butyl-3-methylimidazolium chloride (BmimCl)]. Firstly, the influence of BmimCl on nanopore detection is discussed. It is found that a certain amount of strong polar ionic liquids will disturb the charge distribution in nanopores and increase the detection noise. Then, by analysis of the characteristic current signal of the conical nanopore, the motion behaviour of starch near the entrance of the nanopore was studied and analysis the dominant ion of starch in the BmimCl dissolution process. Finally, based on nuclear magnetic resonance (NMR) and Fourier transform infrared (FTIR) spectroscopy simply discussed the mechanism of amylose and amylopectin dissolved in BmimCl. These results confirm that branched chain structure would affect the dissolution of polysaccharides in ionic liquids and the contribution of anions to the dissolution of polysaccharides are dominant. It is further proved that the current signal can be used to judge the charge and structure information of the analyte, and the dissolution mechanism can be assist analyzed at the single molecule level.

Keywords: Glass nanopore; Ionic liquids; Starch.

MeSH terms

Amylopectin
Ionic Liquids* / chemistry
Magnetic Resonance Spectroscopy
Nanopores*
Starch / chemistry

Substances

Ionic Liquids
Starch
Amylopectin