Correlation between signal transducer and activator of transcription 1 (STAT 1), vascular endothelial growth factor (VEGF), and signal to noise ratio (SNR) value in otoacoustic emission (OAE) examination on organ of Corti cochlea due to cisplatin

Nelfi Disya Amalia Lubis; Tengku Siti Hajar Haryuna; Khalisanni Khalid; Rahadian Zainul

doi:10.17392/1554-23

Correlation between signal transducer and activator of transcription 1 (STAT 1), vascular endothelial growth factor (VEGF), and signal to noise ratio (SNR) value in otoacoustic emission (OAE) examination on organ of Corti cochlea due to cisplatin

Med Glas (Zenica). 2023 Aug 1;20(2). doi: 10.17392/1554-23. Online ahead of print.

Authors

Nelfi Disya Amalia Lubis¹, Tengku Siti Hajar Haryuna¹, Khalisanni Khalid², Rahadian Zainul³

Affiliations

¹ Department of Otorhinolaryngology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Sumatera Utara, Indonesia.
² Malaysian Agricultural Research and Development Institute, Selangor, Malaysia.
³ Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang, Padang, Indonesia.

PMID: 37018057
DOI: 10.17392/1554-23

Abstract

Aim To assess the cochlear damage caused by cisplatin in the rat cochlea based on decreased signal to noise ratio (SNR) values on otoacoustic emission (OAE) examination and increased expression of signal transducer and activator of transcription 1 (STAT 1) and vascular endothelial growth factor (VEGF) on immunohistochemical examination. Methods Twenty-four Rattus norvegicus were divided into 4 groups and injected with 8 mg/kgBW of cisplatin intraperitoneally except for control group. The SNR on OAE examination were checked before the treatment and on day 3, 4, and 7 after the treatment. The cochleas were stained immunohistochemically, followed by assessment of the cochlear organ of Corti damage based on STAT 1 and VEGF expression. Results A decrease in the mean of SNR value was found in accordance with the length of cisplatin exposure. The STAT 1 and VEGF expression increased with duration of cisplatin exposure. A correlation was found between SNR values, STAT 1, and also VEGF expression (p<0.05). Conclusion An increase of STAT 1 and VEGF expression influences cochlear damage due to cisplatin administration. There was a correlation between STAT 1 and VEGF expression with SNR values in the cochlear organ of Corti of Rattus norvegicus exposed to cisplatin.

Keywords: apoptosis; experimental; immunohistochemistry; rat.