Effects of perfluoroalkyl substances on the operational efficiency, microbial communities, and key metabolic pathways of constructed rapid infiltration system with coke as filler layer

Xinjuan Li; Xian Huang; Shuo Fan; Chengyuan Su; Fengxiu Ding; Shitong Wen; Daoning Li; Menglin Chen

doi:10.1016/j.biortech.2023.128998

Effects of perfluoroalkyl substances on the operational efficiency, microbial communities, and key metabolic pathways of constructed rapid infiltration system with coke as filler layer

Bioresour Technol. 2023 Jun:378:128998. doi: 10.1016/j.biortech.2023.128998. Epub 2023 Apr 1.

Authors

Xinjuan Li¹, Xian Huang¹, Shuo Fan¹, Chengyuan Su², Fengxiu Ding¹, Shitong Wen¹, Daoning Li¹, Menglin Chen¹

Affiliations

¹ Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, 15 Yucai Road, Guilin 541004, PR China.
² Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, 15 Yucai Road, Guilin 541004, PR China; College of Environment and Resources, Guangxi Normal University, 15 Yucai Road, Guilin 541004, PR China. Electronic address: suchengyuan2008@126.com.

PMID: 37011846
DOI: 10.1016/j.biortech.2023.128998

Abstract

Influences of perfluoroalkyl substances on the performance and microbial metabolic pathways of constructed rapid infiltration systems are not fully understood. In this study, wastewater containing different concentrations of perfluorooctanoic acid (PFOA)/perfluorobutyric acid (PFBA) was treated in constructed rapid infiltration systems with coke as filler. The addition of 5 and 10 mg/L PFOA inhibited the removal of chemical oxygen demand (COD) (80.42%, 89.27%), ammonia nitrogen (31.32%, 41.14%), and total phosphorus (TP) (43.30%, 39.34%). Meanwhile, 10 mg/L PFBA inhibited TP removal of the systems. Based on X-ray photoelectron spectroscopy, the percentages of F^- within the PFOA and PFBA groups were 12.91% and 48.46%, respectively. PFOA transformed Proteobacteria (71.79%) into the dominant phyla of the systems, whereas PFBA enriched Actinobacteria (72.51%). The PFBA up-regulated the coding gene of 6-phosphofructokinase by 14.44%, whereas PFOA down-regulated it by 4.76%. These findings provide insights into the toxicity of perfluoroalkyl substances on constructed rapid infiltration systems.

Keywords: Coke; Constructed rapid infiltration; Key metabolic pathways; PFBA; PFOA.

MeSH terms

Coke*
Fluorocarbons* / analysis
Fluorocarbons* / chemistry
Microbiota*
Phosphorus
Wastewater
Water Pollutants, Chemical*

Substances

Coke
Wastewater
perfluorooctanoic acid
Fluorocarbons
perfluorobutyric acid
Phosphorus
Water Pollutants, Chemical