Purification and characterization of the enzymes from Brevundimonas naejangsanensis that degrade ochratoxin A and B

Mengxue Peng; Zhenzhen Zhang; Xinge Xu; Haoxiang Zhang; Zitong Zhao; Zhihong Liang

doi:10.1016/j.foodchem.2023.135926

Purification and characterization of the enzymes from Brevundimonas naejangsanensis that degrade ochratoxin A and B

Food Chem. 2023 Sep 1:419:135926. doi: 10.1016/j.foodchem.2023.135926. Epub 2023 Mar 20.

Authors

Mengxue Peng¹, Zhenzhen Zhang¹, Xinge Xu¹, Haoxiang Zhang¹, Zitong Zhao¹, Zhihong Liang²

Affiliations

¹ College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China.
² College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China; The Supervision, Inspection and Testing Center of Genetically Modified Organisms, Ministry of Agriculture, Beijing, China; Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China. Electronic address: lzh105@cau.edu.cn.

PMID: 37011575
DOI: 10.1016/j.foodchem.2023.135926

Abstract

Ochratoxin A (OTA) and Ochratoxin B (OTB) co-contaminate many types of agricultural products. Screening enzymes that degrade both OTA and OTB has significance in food safety. In this study, four novel OTA and OTB degrading enzymes, namely BnOTase1, BnOTase2, BnOTase3, and BnOTase4, were purified from the metabolites of the Brevundimonas naejangsanensis ML17 strain. These four enzymes hydrolyzed OTA into OTα and hydrolyzed OTB into OTβ. BnOTase1, BnOTase2, BnOTase3, and BnOTase4 have the apparent K_m values for hydrolyzing OTA of 19.38, 0.92, 12.11, 1.09 μmol/L and for hydrolyzing OTB of 0.76, 2.43, 0.60, 0.64 μmol/L respectively. OTα and OTβ showed no significant cytotoxicity to HEK293 cells, suggesting that these enzymes mitigate the toxicity of OTA and OTB. The discovery of the novel OTA and OTB degrading enzymes enriches the research on ochratoxin control and provides objects for protein rational design.

Keywords: Amidase; Biodegradation; Brevundimonas naejangsanensis; Ochratoxin A; Ochratoxin B; Peptidase.

MeSH terms

Caulobacteraceae / chemistry
Caulobacteraceae / metabolism
HEK293 Cells
Humans
Ochratoxins*

Substances

ochratoxin A
ochratoxin B
Ochratoxins

Supplementary concepts

Brevundimonas naejangsanensis