Identification of LSM family members as potential chemoresistance predictive and therapeutic biomarkers for gastric cancer

Qianhui Liu; Qinghai Lian; Yingqiu Song; Shangbin Yang; Changchang Jia; Jiafeng Fang

doi:10.3389/fonc.2023.1119945

Identification of LSM family members as potential chemoresistance predictive and therapeutic biomarkers for gastric cancer

Front Oncol. 2023 Mar 17:13:1119945. doi: 10.3389/fonc.2023.1119945. eCollection 2023.

Authors

Qianhui Liu¹, Qinghai Lian², Yingqiu Song¹, Shangbin Yang¹, Changchang Jia², Jiafeng Fang¹

Affiliations

¹ Department of Gastrointestinal Surgery, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
² Department of Cell-Gene Therapy Translational Medicine Research Center, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China.

Abstract

Introduction: The Like-Smith (LSM) family plays a critical role in the progression of several cancers. However, the function of LSMs in chemoresistance of gastric cancer (GC) is still elusive.

Methods: The Cancer Genome Atlas (TCGA) database, Gene Expression Omnibus (GEO) database and Tumor Immune Estimation Resource Analysis (TIMER) were utilized to analyze the expression, prognostic value and immune infiltration of LSMs in GC patients. Moreover, qPCR and immunohistochemistry (IHC) experiment were conducted with clinical samples.

Results: The expression of LSMs was upregulated in GC tissues and most of LSMs were negatively correlated with overall survival of GC patients with 5-fluorouracil (5-FU) treatment. We further revealed that LSM5, 7 and 8 were hub genes of GEO (GSE14210). Besides, the qPCR results demonstrated that a higher level of LSM5 and LSM8 was associated with 5-FU chemoresistance in GC. Moreover, both TIMER and IHC revealed that a lower expression of LSM5 and LSM8 was correlated with high infiltration of T cells, regulatory T cells, B cells, macrophages, and neutrophils.

Discussion: Our study systematically investigated the expression pattern and biological features of LSM family members in GC, and identified LSM5 and LSM8 as potential biomarkers in GC with 5-FU chemotherapy.

Keywords: 5-fluorouracil; LSM family; chemotherapy resistance; gastric cancer; immune infiltration; prognosis.

Grants and funding

This work was supported by National Natural Science Foundation of China (No. 81901471 to JF; No. 81402426 to CJ); Natural Science Foundation of Guangdong Province (No. 2021A1515010577 to JF; No. 2022A1515012653 to JF; No. 2022A1515012650 to CJ); The Cultivation Project of National Natural Science Foundation of the Third Affiliated Hospital of Sun Yat-sen University (No. 2020GZRPYMS09 to CJ).