Protective effects of extracts from Acer truncatum leaves on SLS-induced HaCaT cells

Yanxiao Fan; Ronghui Gu; Ruifei Zhang; Miaomiao Wang; Heran Xu; Min Wang; Chunlin Long

doi:10.3389/fphar.2023.1068849

Protective effects of extracts from Acer truncatum leaves on SLS-induced HaCaT cells

Front Pharmacol. 2023 Mar 15:14:1068849. doi: 10.3389/fphar.2023.1068849. eCollection 2023.

Authors

Yanxiao Fan^{1

2}, Ronghui Gu^{3

4}, Ruifei Zhang^{1

2}, Miaomiao Wang^{1

2}, Heran Xu^{5

6}, Min Wang^{5

6}, Chunlin Long^{1

2

7

8}

Affiliations

¹ Key Laboratory of Ecology and Environment in Minority Areas (Minzu University of China), National Ethnic Affairs Commission, Beijing, China.
² College of Life and Environmental Sciences, Minzu University of China, Beijing, China.
³ Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Guizhou University), Ministry of Education, Guiyang, China.
⁴ School of Liquor and Food Engineering, Guizhou University, Guiyang, China.
⁵ College of Chemistry and Materials Engineering, Beijing Technology and Business University, Beijing, China.
⁶ BTBU-TANGYI Innovation Center for the Evaluation of the Safety and Efficacy of Bioengineering Raw Materials, Beijing, China.
⁷ Key Laboratory of Ethnomedicine (Minzu University of China), Ministry of Education, Beijing, China.
⁸ Institute of National Security Studies, Minzu University of China, Beijing, China.

Abstract

Introduction: A. truncatum Bunge (Sapindaceae or formerly Aceraceae) is a tall deciduous tree native to China. Traditionally, the leaves of A. truncatum are decocted and used by Chinese Mongolians, Koreans, and Tibetans to treat skin itching, dry cracks, and other skin ailments, which indicates A. truncatum leaves may have a potential inhibitory effect on various skin inflammations. Methods: To examine the protective effect against skin inflammations of A. truncatum leaf extract (ATLE), an in vitro dermatitis model was established using sodium dodecyl sulfate (SLS)-induced HaCaT cells. The anti-inflammatory effect of ATLE was evaluated by analyzing cell viability, apoptosis, reactive oxygen species (ROS), interleukin 6 (IL-6), and prostaglandin E2 (PGE2) levels. Results: Orthogonal experiments showed that the pretreatment with ATLE can reduce the IL-6 levels, PGE2 levels, and apoptosis increased in SLS-stimulated HaCaT cells, which indicates that ATLE has positive efficacy for dermatitis. Furthermore, three flavonoid compounds kaempferol-3-O-α-L-rhamnoside, quercetin-3-O-α-L-rhamnopyranoside, kaempferol-3,7-di-O-α-L-rhamnoside, and 1,2,3,4,6-Penta-O-galloyl-β-D-glucopyranose (PGG) were isolated and identified. Among them, kaempferol-3,7-di-O-α-L-rhamnoside was isolated from this plant for the first time. These compounds have been proven to have an anti-inflammatory effect. They may contribute to the efficacy of A. truncatumin treating skin inflammation. Discussion: The results revealed that ATLE has the potential to be used as an additive in various skin care products to prevent skin inflammations and may be incorporated in formulations for topical application as a therapeutic approach against dermatitis.

Keywords: A. truncatum; dermatitis; flavonoids; interleukin 6; skin inflammations.

Grants and funding

This study was financially supported by the National Natural Science Foundation of China (31761143001 and 31870316), Minzu University of China (2020MDJC03, 2022ZDPY10 and 2022GJAQ04), and First-class Discipline Construction Project in Guizhou Province (GDTGHZ2020003).