First record of Perkinsus marinus infecting Crassostrea sp. in Rio Grande do Norte, Brazil, using real-time PCR

Célio Souza da Rocha; Rachel Costa Sabry; Rafael Dos Santos Rocha; Rodrigo Maggioni; Bruno Vinicius Silva de Araújo; Renata Julia Dos Santos Silva; João Marcelo Azevedo de Paula Antunes

doi:10.1016/j.jip.2023.107917

First record of Perkinsus marinus infecting Crassostrea sp. in Rio Grande do Norte, Brazil, using real-time PCR

J Invertebr Pathol. 2023 Jun:198:107917. doi: 10.1016/j.jip.2023.107917. Epub 2023 Mar 31.

Authors

Célio Souza da Rocha¹, Rachel Costa Sabry², Rafael Dos Santos Rocha³, Rodrigo Maggioni⁴, Bruno Vinicius Silva de Araújo⁵, Renata Julia Dos Santos Silva⁶, João Marcelo Azevedo de Paula Antunes⁷

Affiliations

¹ Universidade Federal Rural do Semi-Árido, Av. Francisco Mota, 572 - Costa e Silva, Mossoró, RN, Postal Code: 59625-900, Brazil. Electronic address: celiorocha.vet@hotmail.com.
² Instituto Federal de Educação Ciência e Tecnologia do Ceará, Campus Aracati, Rodovia CE-040 km 139, Postal Code: 62800-000, Aracati, CE, Brazil. Electronic address: rachelsabry@ifce.edu.br.
³ Instituto de Ciências do Mar, Universidade Federal do Ceará, Av. da Abolição, 3207 - Meireles, Postal Code: 60165-081, Fortaleza, CE, Brazil. Electronic address: rafaelsanro@ufc.br.
⁴ Instituto de Ciências do Mar, Universidade Federal do Ceará, Av. da Abolição, 3207 - Meireles, Postal Code: 60165-081, Fortaleza, CE, Brazil. Electronic address: maggioni@ufc.br.
⁵ Universidade Federal Rural do Semi-Árido, Av. Francisco Mota, 572 - Costa e Silva, Mossoró, RN, Postal Code: 59625-900, Brazil. Electronic address: brunovinicios.araujo@hotmail.com.
⁶ Instituto Federal de Educação Ciência e Tecnologia do Ceará, Campus Aracati, Rodovia CE-040 km 139, Postal Code: 62800-000, Aracati, CE, Brazil. Electronic address: renatajulia709@gmail.com.
⁷ Universidade Federal Rural do Semi-Árido, Av. Francisco Mota, 572 - Costa e Silva, Mossoró, RN, Postal Code: 59625-900, Brazil. Electronic address: joao.antunes@ufersa.edu.br.

PMID: 37004916
DOI: 10.1016/j.jip.2023.107917

Abstract

A pathogen with high virulence potential in some host species, Perkinsus marinus remains a challenge for the ecological integrity of marine ecosystems and the health of bivalve molluscs. This study investigates the occurrence of P. marinus in Crassostrea sp. in estuaries of the Potengi River and the Guaraíras lagoon in Rio Grande do Norte, Brazil. A total of 203 oyster samples that tested positive for Perkinsus sp. in Ray's fluid thioglycollate medium (RFTM) were subjected to species-specific quantitiative PCR, where 61 animals (30.05 %) presented amplification graphs with a melting temperature of 80.1 ± 0.6 °C matching the positive control. This was the first record of P. marinus in oysters in these estuaries using qPCR as a diagnostic tool.

Keywords: Crassostrea sp.; Perkinsus marinus; qPCR.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Apicomplexa* / genetics
Brazil
Crassostrea*
Ecosystem
Real-Time Polymerase Chain Reaction