Photoacoustic microscopy (PAM) is a high-resolution imaging modality that has been mainly implemented with small field of view applications. Here, we developed a fast PAM system that utilizes a unique spiral laser scanning mechanism and a wide acoustic detection unit. The developed system can image an area of 12.5 cm2 in 6.4 s. The system has been characterized using highly detailed phantoms. Finally, the imaging capabilities of the system were further demonstrated by imaging a sheep brain ex vivo and a rat brain in vivo.
Keywords: fast imaging; large field of view; photoacoustic microscopy; spiral scanning.
© 2023 The Authors. Journal of Biophotonics published by Wiley-VCH GmbH.