Rapid determination of SARS-CoV-2 antibody neutralization titer using Bio-Rad Bio-Plex correlates strongly with pseudovirus-determined neutralization titer

J Virol Methods. 2023 Jun:316:114726. doi: 10.1016/j.jviromet.2023.114726. Epub 2023 Mar 28.

Abstract

Accurate and rapid evaluation of SARS-CoV-2 half-maximal neutralizing antibody (nAb) titer (NT50) is an important research tool for measuring nAb responses after prophylaxis or therapeutics for COVID-19 prevention and management. Compared with ACE2-competitive enzyme immunoassays for nAb detection, pseudovirus assays remain low-throughput and labor intensive. A novel application of the Bio-Rad Bio-Plex Pro Human SARS-CoV-2 D614G S1 Variant nAb Assay was used to determine NT50 from COVID-19-vaccinated individuals and showed strong correlation to a laboratory-developed SARS-CoV-2 pseudovirus nAb assay. The Bio-Plex nAb assay could provide a rapid, high-throughput, culture-free method for NT50 determination in sera.

Keywords: ACE2 competitive assay; Bio-Plex; Neutralizing antibody; Neutralizing titer (NT50); Pseudovirus assay; SARS-CoV-2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angiotensin-Converting Enzyme 2
  • Antibodies, Neutralizing
  • Antibodies, Viral
  • COVID-19* / diagnosis
  • Humans
  • RNA Viruses*
  • SARS-CoV-2

Substances

  • Antibodies, Viral
  • Antibodies, Neutralizing
  • Angiotensin-Converting Enzyme 2

Supplementary concepts

  • SARS-CoV-2 variants