Efficient Targeted Mutagenesis in Brassica Crops Using CRISPR/Cas Systems

Tom Lawrenson; Mark Youles; Monika Chhetry; Martha Clarke; Wendy Harwood; Penny Hundleby

doi:10.1007/978-1-0716-3131-7_16

Efficient Targeted Mutagenesis in Brassica Crops Using CRISPR/Cas Systems

Methods Mol Biol. 2023:2653:253-271. doi: 10.1007/978-1-0716-3131-7_16.

Authors

Tom Lawrenson¹, Mark Youles², Monika Chhetry², Martha Clarke², Wendy Harwood², Penny Hundleby²

Affiliations

¹ John Innes Centre, Norwich, Norfolk, UK. tom.lawrenson@JIC.ac.uk.
² John Innes Centre, Norwich, Norfolk, UK.

PMID: 36995631
DOI: 10.1007/978-1-0716-3131-7_16

Abstract

CRISPR/Cas has been established for targeted mutagenesis in many plant species since 2013, including Brassica napus and Brassica oleracea. Since that time, improvements have been made in terms of efficiency and choice of CRISPR systems. This protocol encompasses improved Cas9 efficiency and an alternative Cas12a system, allowing more challenging and diverse editing outcomes to be achieved.

Keywords: Brassica napus; Brassica oleracea; CRISPR/Cas9; Cas12a; Genome editing; Targeted mutagenesis.

MeSH terms

Brassica napus* / genetics
Brassica* / genetics
CRISPR-Cas Systems / genetics
Gene Editing / methods
Mutagenesis