Sphere-Based Expansion of Myogenic Progenitors from Human Pluripotent Stem Cells

Megan Reilly; Samantha Robertson; Masatoshi Suzuki

doi:10.1007/978-1-0716-3036-5_12

Sphere-Based Expansion of Myogenic Progenitors from Human Pluripotent Stem Cells

Methods Mol Biol. 2023:2640:159-174. doi: 10.1007/978-1-0716-3036-5_12.

Authors

Megan Reilly¹, Samantha Robertson¹, Masatoshi Suzuki²

Affiliations

¹ Department of Comparative Biosciences, The Stem Cell and Regenerative Medicine Center, University of Wisconsin, Madison, WI, USA.
² Department of Comparative Biosciences, The Stem Cell and Regenerative Medicine Center, University of Wisconsin, Madison, WI, USA. masatoshi.suzuki@wisc.edu.

PMID: 36995594
DOI: 10.1007/978-1-0716-3036-5_12

Abstract

The protocol presented here is to derive, maintain, and differentiate human pluripotent stem cells into skeletal muscle progenitor/stem cells (myogenic progenitors) using a sphere-based culture approach. This sphere-based culture is an attractive method for maintaining progenitor cells due to their longevity and the presence of cell-cell interactions and molecules. Large numbers of cells can be expanded in culture using this method, which represents a valuable source for cell-based tissue modeling and regenerative medicine.

Keywords: EZ spheres; Human embryonic stem cells; Human induced pluripotent stem cells; Human pluripotent stem cells; Myogenic progenitors; Skeletal muscle; Skeletal myotubes.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation
Humans
Induced Pluripotent Stem Cells*
Muscle Development
Muscle Fibers, Skeletal
Muscle, Skeletal
Pluripotent Stem Cells*
Satellite Cells, Skeletal Muscle*

Abstract

Publication types

MeSH terms

Grants and funding