ALDH2 attenuates myocardial pyroptosis through breaking down Mitochondrion-NLRP3 inflammasome pathway in septic shock

Ying Zhang; Ying Lv; Qingju Zhang; Xingfang Wang; Qi Han; Yan Liang; Simeng He; Qiuhuan Yuan; Jiaqi Zheng; Changchang Xu; Xiangxin Zhang; Zichen Wang; Huaxiang Yu; Li Xue; Jiali Wang; Feng Xu; Jiaojiao Pang; Yuguo Chen

doi:10.3389/fphar.2023.1125866

ALDH2 attenuates myocardial pyroptosis through breaking down Mitochondrion-NLRP3 inflammasome pathway in septic shock

Front Pharmacol. 2023 Mar 13:14:1125866. doi: 10.3389/fphar.2023.1125866. eCollection 2023.

Authors

Ying Zhang^{1

2

3

4}, Ying Lv^{1

2

3

4}, Qingju Zhang^{1

2

3

4}, Xingfang Wang^{1

2

3

4}, Qi Han^{1

2

3

4}, Yan Liang^{1

2

3

4}, Simeng He^{1

2

3

4}, Qiuhuan Yuan^{1

2

3

4}, Jiaqi Zheng^{1

2

3

4}, Changchang Xu^{1

2

3

4}, Xiangxin Zhang^{1

2

3

4}, Zichen Wang^{1

2

3

4}, Huaxiang Yu^{1

2

3

4}, Li Xue^{1

2

3

4}, Jiali Wang^{1

2

3

4}, Feng Xu^{1

2

3

4}, Jiaojiao Pang^{1

2

3

4}, Yuguo Chen^{1

2

3

4}

Affiliations

¹ Department of Emergency Medicine, Qilu Hospital of Shandong University, Jinan, China.
² Chest Pain Center, Shandong Provincial Clinical Research Center for Emergency and Critical Care Medicine, Institute of Emergency and Critical Care Medicine of Shandong University, Qilu Hospital of Shandong University, Jinan, China.
³ Key Laboratory of Emergency and Critical Care Medicine of Shandong Province, Key Laboratory of Cardiopulmonary-Cerebral Resuscitation Research of Shandong Province, Shandong Provincial Engineering Laboratory for Emergency and Critical Care Medicine, Qilu Hospital of Shandong University, Jinan, China.
⁴ The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education, Chinese Ministry of Health and Chinese Academy of Medical Sciences, The State and Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Qilu Hospital of Shandong University, Jinan, China.

Abstract

Cell survival or death is critical for cardiac function. Myocardial pyroptosis, as a newly recognized programmed cell death, remains poorly understood in sepsis. In this study, we evaluated the effect of aldehyde dehydrogenase (ALDH2) on myocardial pyroptosis and revealed the underlying mechanisms in sepsis. We established a septic shock mice model by intraperitoneal injection of Lipopolysaccharide (LPS, 15 mg/kg) 12 h before sacrifice. It was found that aldehyde dehydrogenase significantly inhibited NOD-like receptor protein 3 (NLRP3) inflammasome activation and Caspase-1/GSDMD-dependent pyroptosis, which remarkably improved survival rate and septic shock-induced cardiac dysfunction, relative to the control group. While aldehyde dehydrogenase knockout or knockdown significantly aggravated these phenomena. Intriguingly, we found that aldehyde dehydrogenase inhibited LPS-induced deacetylation of Hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex α subunit (HADHA) by suppressing the translocation of Histone deacetylase 3 (HDAC3) from nuclei to mitochondria. Acetylated HADHA is essential for mitochondrial fatty acid β-oxidation, and its interruption can result in accumulation of toxic lipids, induce mROS and cause mtDNA and ox-mtDNA release. Our results confirmed the role of Histone deacetylase 3 and HADHA in NOD-like receptor protein 3 inflammasome activation. Hdac3 knockdown remarkedly suppressed NOD-like receptor protein 3 inflammasome and pyroptosis, but Hadha knockdown eliminated the effect. aldehyde dehydrogenase inhibited the translocation of Histone deacetylase 3, protected ac-HADHA from deacetylation, and significantly reduced the accumulation of toxic aldehyde, and inhibited mROS and ox-mtDNA, thereby avoided NOD-like receptor protein 3 inflammasome activation and pyroptosis. This study provided a novel mechanism of myocardial pyroptosis through mitochondrial Histone deacetylase 3/HADHA- NOD-like receptor protein 3 inflammasome pathway and demonstrated a significant role of aldehyde dehydrogenase as a therapeutic target for myocardial pyroptosis in sepsis.

Keywords: HADHA; NLRP3 inflammasome; aldehyde dehydrogenase 2; myocardial pyroptosis; septic shock.

Grants and funding

This study was supported by the State Key Program of the National Natural Science Foundation of China (82030059), National Natural Science Foundation of China (82172127, 81772036, 82072144, 81671952, 81873950, 81873953), National Key R&D Program of China (2020YFC1512700, 2020YFC1512705, 2020YFC1512703, 2020YFC0846600), National S&T Fundamental Resources Investigation Project (2018FY100600, 2018FY100602), Taishan Pandeng Scholar Program of Shandong Province (tspd20181220), Taishan Young Scholar Program of Shandong Province (tsqn20161065, tsqn201812129), Youth Top-Talent Project of National Ten Thousand Talents Plan, Qilu Young Scholar Program and the Fundamental Research Funds of Shandong University (2018JC011).