Genome Characterisation of the CGMMV Virus Population in Australia-Informing Plant Biosecurity Policy

Joanne Mackie; Paul R Campbell; Monica A Kehoe; Lucy T T Tran-Nguyen; Brendan C Rodoni; Fiona E Constable

doi:10.3390/v15030743

Genome Characterisation of the CGMMV Virus Population in Australia-Informing Plant Biosecurity Policy

Viruses. 2023 Mar 14;15(3):743. doi: 10.3390/v15030743.

Authors

Joanne Mackie^{1

2}, Paul R Campbell³, Monica A Kehoe⁴, Lucy T T Tran-Nguyen⁵, Brendan C Rodoni^{1

2}, Fiona E Constable^{1

2}

Affiliations

¹ School of Applied Systems Biology, La Trobe University, Melbourne, VIC 3083, Australia.
² Agriculture Victoria Research, Department of Energy, Environment and Climate Action, Melbourne, VIC 3083, Australia.
³ Horticulture and Forestry Science, Department of Agriculture and Fisheries, Ecosciences Precinct, Brisbane, QLD 4102, Australia.
⁴ Biosecurity and Sustainability, Department of Primary Industries and Regional Development, South Perth, WA 6151, Australia.
⁵ Plant Health Australia, Canberra, ACT 2600, Australia.

Abstract

The detection of cucumber green mottle mosaic (CGMMV) in the Northern Territory (NT), Australia, in 2014 led to the introduction of strict quarantine measures for the importation of cucurbit seeds by the Australian federal government. Further detections in Queensland, Western Australia (WA), New South Wales and South Australia occurred in the period 2015-2020. To explore the diversity of the current Australian CGMMV population, 35 new coding sequence complete genomes for CGMMV isolates from Australian incursions and surveys were prepared for this study. In conjunction with published genomes from the NT and WA, sequence, phylogenetic, and genetic variation and variant analyses were performed, and the data were compared with those for international CGMMV isolates. Based on these analyses, it can be inferred that the Australian CGMMV population resulted from a single virus source via multiple introductions.

Keywords: cucumber green mottle mosaic virus; genetic diversity; median-joining network.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biosecurity
Citrullus*
Cucumis sativus*
Northern Territory
Phylogeny
Plant Diseases / prevention & control
Tobamovirus* / genetics

Supplementary concepts

Cucumber green mottle mosaic virus

Grants and funding

This project has been funded by Hort Innovation Australia Limited (VG16086) using vegetable industry levies with co-investment from the Queensland Department of Agriculture and Fisheries; the Victorian Department of Jobs, Precincts and Regions; the Northern Territory Department of Industry, Tourism and Trade; the Western Australia Department of Primary Industries and Regional Development; and the University of Tasmania. Hort Innovation is the grower-owned, not-for-profit research and development corporation for Australian horticulture. This research was conducted using the facilities of Agriculture Victoria.