Construction of bacterial laccase displayed on the microbial surface for ultrasensitive biosensing of phenolic pollutants with nanohybrids-enhanced performance

Abstract

Although bacterial laccase (BLac) has many advantages including short fermentation period and adaptable activity to wide temperature and pH ranges, it is of challenge and significance to apply BLac to the biosensors, due to the intracellular secretion and poor electron transfer efficiency of BLac. Here, cell surface-displayed BLac (CSDBLac) was successfully constructed as whole-cell biocatalyst through microbial surface display technology, eliminating the mass transfer restriction and laborious purification steps. Meanwhile, MXenes/polyetherimide-multiwalled carbon nanotubes (MXenes/PEI-MWCNTs) nanohybrids were designed to immobilize CSDBLac and improve their electrochemical activity. Then, an electrochemical biosensor was successfully constructed to detect common phenolic pollutants (catechol and hydroquinone) by the co-immobilization of CSDBLac and MXenes/PEI-MWCNTs nanohybrids onto a glassy carbon electrode. Subsequently, it was successfully applied to the water samples assay with good reliability and repeatability. This work innovatively used BLac and nanohybrid as the core elements of biosensor, which not only effectively solved the application bottleneck of BLac on biosensors, but also dramatically promote the electro transfer efficiency between whole-cell biocatalyst and electrode. This method is of profound meanings for significantly improving the performance of phenolic biosensors and other biosensors from the origin.

Keywords: Bacterial laccase; Biosensor; Microbial surface display; Nanomaterial; Phenolic pollutant.