Construction of a water-soluble fluorescent probe for copper (II) ion detection in live cells and food products

Food Chem. 2023 Aug 30:418:135994. doi: 10.1016/j.foodchem.2023.135994. Epub 2023 Mar 23.

Abstract

The quality of wine can be affected by excess Cu2+ due to the occurrence of oxidation reactions or precipitation. Therefore, it is essential to use simple and effective testing methods to ensure the Cu2+ content in wine. In this work, we designed and synthesized a rhodamine polymer fluorescent probe (PEG-R). The water solubility of PEG-R was improved by the introduction of polyethylene glycol, which improved the performance and broadened its application in the food field. The PEG-R was characterized by high sensitivity, selectivity and fast response to Cu2+ and was able to complete the response process within 30 s, with approximately 29-fold fluorescence enhancement of the probe after exposure to Cu2+, the limit of detection (LOD) was 1.295 × 10-6 M. The probe can be used for the determination of Cu2+ in living cells, zebrafish, white wine and food products, and it was made into practical gels and test strips.

Keywords: Copper (Ⅱ); Fluorescence imaging; Food products; Polyethylene glycol; Rhodamine; White wine.

MeSH terms

  • Animals
  • Cell Survival
  • Copper*
  • Fluorescent Dyes*
  • Food
  • Rhodamines
  • Spectrometry, Fluorescence
  • Water
  • Zebrafish

Substances

  • Copper
  • Fluorescent Dyes
  • Rhodamines
  • Water