Targeted HPTLC Profile, Quantification of Flavonoids and Phenolic Acids, and Antimicrobial Activity of Dodonaea angustifolia (L.f.) Leaves and Flowers

Fekade Beshah Tessema; Yilma Hunde Gonfa; Tilahun Belayneh Asfaw; Mesfin Getachew Tadesse; Tigist Getachew Tadesse; Archana Bachheti; Mohammed O Alshaharni; Pankaj Kumar; Vinod Kumar; Ivan Širić; Sami Abou Fayssal; Kundan Kumar Chaubey; Rakesh Kumar Bachheti

doi:10.3390/molecules28062870

Targeted HPTLC Profile, Quantification of Flavonoids and Phenolic Acids, and Antimicrobial Activity of Dodonaea angustifolia (L.f.) Leaves and Flowers

Molecules. 2023 Mar 22;28(6):2870. doi: 10.3390/molecules28062870.

Authors

Fekade Beshah Tessema^{1

2

3}, Yilma Hunde Gonfa^{1

2

4}, Tilahun Belayneh Asfaw^{1

5}, Mesfin Getachew Tadesse^{1

2}, Tigist Getachew Tadesse⁶, Archana Bachheti⁷, Mohammed O Alshaharni⁸, Pankaj Kumar⁹, Vinod Kumar⁹, Ivan Širić¹⁰, Sami Abou Fayssal^{11

12}, Kundan Kumar Chaubey¹³, Rakesh Kumar Bachheti^{1

2}

Affiliations

¹ Department of Industrial Chemistry, Addis Ababa Science and Technology University, Addis Ababa P.O. Box 16417, Ethiopia.
² Centre of Excellence in Biotechnology and Bioprocess, Addis Ababa Science and Technology University, Addis Ababa P.O. Box 16417, Ethiopia.
³ Department of Chemistry, Faculty of Natural and Computational Science, Woldia University, Woldia P.O. Box. 400, Ethiopia.
⁴ Department of Chemistry, Faculty of Natural and Computational Science, Ambo University, Ambo P.O. Box 19, Ethiopia.
⁵ Department of Chemistry, College of Natural and Computational Science, Gondar University, Gondar P.O. Box 196, Ethiopia.
⁶ Bio and Emerging Technology Institute, Health Biotechnology Directorate, Addis Ababa P.O. Box 5954, Ethiopia.
⁷ Department of Environment Science, Graphic Era (Deemed to be University), Dehradun 248002, India.
⁸ Department of Biology, College of Science, King Khalid University, Abha 61321, Saudi Arabia.
⁹ Agro-Ecology and Pollution Research Laboratory, Department of Zoology and Environmental Science, Gurukula Kangri (Deemed to Be University), Haridwar 249404, India.
¹⁰ University of Zagreb, Faculty of Agriculture, Svetosimunska 25, 10000 Zagreb, Croatia.
¹¹ Department of Agronomy, Faculty of Agronomy, University of Forestry, 10 Kliment Ohridski Blvd, 1797 Sofia, Bulgaria.
¹² Department of Plant Production, Faculty of Agriculture, Lebanese University, Beirut 1302, Lebanon.
¹³ Division of Research and Innovation, School of Applied and Life Sciences, Uttaranchal University, Arcadia Grant, P.O. Chandanwari, Prem Nagar, Dehradun 248007, India.

Abstract

In East Africa, Dodonaea angustifolia (L.f.) is a well-known medicinal herb. Its leaf is primarily studied in light of its ethnobotanical use. In terms of phytochemistry and biological activity, its flower is not studied. In a prior study, our team looked into phytochemical screening, antioxidant activity, and total phenolic levels. This study aims to compare the profiles and biological activities of the leaf and flower samples of D. angustifolia and to present therapeutic alternatives. The leaf and flower sample powders were extracted with methanol using ultrasound-assisted extraction (UAE). HPTLC profile was obtained using CAMAG-HPTLC equipped with VisionCATS software. Antimicrobial agar well diffusion assay and minimum inhibition concentration (MIC) were determined. The leaf and flower extracts of D. angustifolia showed antibacterial activity with a MIC value of 20 µg/mL against Enterococcus faecalis and Listeria monocytogenes. Similarly, 40 µg/mL was found to be effective against Aspergillus flavus. D. angustifolia flower is a rich source of flavonoids and phenolic acids. Because of its antibacterial properties and profile, which are almost the same, the flower is emerging as a viable option for medicinal alternatives.

Keywords: Dodonaea angustifolia; antimicrobial activities; flavonoids; medicinal plants; phenolic contents.

MeSH terms

Anti-Bacterial Agents / analysis
Anti-Bacterial Agents / pharmacology
Antioxidants / analysis
Antioxidants / pharmacology
Flavonoids* / chemistry
Flowers / chemistry
Plant Extracts / chemistry
Plant Leaves / chemistry
Sapindaceae*

Substances

Flavonoids
Plant Extracts
phenolic acid
Anti-Bacterial Agents
Antioxidants

Abstract

MeSH terms

Substances

Grants and funding