Development of Improved DNA Collection and Extraction Methods for Handled Documents

Genes (Basel). 2023 Mar 21;14(3):761. doi: 10.3390/genes14030761.

Abstract

Handwritten documents may contain probative DNA, but most crime laboratories do not process this evidence. DNA recovery should not impair other evidence processing such as latent prints or indented writing. In this study, single fingermarks on paper were sampled with flocked swabs, cutting, and dry vacuuming. In addition, two extraction methods were compared for the sample type. DNA yields were low across all methods; however, this work confirms the ability to recover DNA from paper and the usefulness of the vacuum sampling method combined with the Chelex-Tween method. Stability of touch DNA deposits were compared over an 11-month period to better understand degradation that may occur over time. No significant difference in DNA recovery was observed, suggesting DNA deposits on paper are stable over an 11-month span.

Keywords: fingermarks; paper evidence; sample collection; touch DNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Crime*
  • DNA / genetics
  • DNA Fingerprinting*
  • Specimen Handling / methods
  • Touch

Substances

  • DNA

Grants and funding

This research was funded by the 2017 Forensic Sciences Foundation Lucas Research Grant.