Sequential extraction, structural characterization, and antioxidant activity of polysaccharides from Dendrocalamus brandisii bamboo shoot shell

Xiaowei Peng; Jingjie Liu; Ni Tang; Jia Deng; Can Liu; Huan Kan; Ping Zhao; Xia Zhang; Zhengjun Shi; Yun Liu

doi:10.1016/j.fochx.2023.100621

Sequential extraction, structural characterization, and antioxidant activity of polysaccharides from Dendrocalamus brandisii bamboo shoot shell

Food Chem X. 2023 Mar 5:17:100621. doi: 10.1016/j.fochx.2023.100621. eCollection 2023 Mar 30.

Authors

Xiaowei Peng¹, Jingjie Liu¹, Ni Tang¹, Jia Deng², Can Liu¹, Huan Kan², Ping Zhao^{1

2}, Xia Zhang³, Zhengjun Shi¹, Yun Liu^{1

2}

Affiliations

¹ Key Laboratory of State Forestry and Grassland Administration on Highly-Efficient Utilization of Forestry Biomass Resources in Southwest China, Southwest Forestry University, Kunming 650224, China.
² Key Laboratory of Forest Resources Conservation and Utilization in the Southwest Mountains of China Ministry of Education, Southwest Forestry University, Kunming 650224, China.
³ School of Pharmacy, Guizhou University, Guiyang 550025, China.

Abstract

Polysaccharides including water-soluble fraction (W), 1,2-Cyclohexanediaminetetraacetic acid (CDTA)-soluble fraction (CA), sodium carbonate (Na₂CO₃)-soluble fraction (SC), 1 M potassium hydroxide (KOH)-soluble fractions (PH1), and 4 M KOH-soluble fraction (PH4) were successively extracted from Dendrocalamus brandisii bamboo shoot shells using water, CDTA, Na₂CO₃, and KOH solution. The analytical methods were employed to initially identify the structural characteristics of the five polysaccharide fractions, and their antioxidant capacities in vitro were determined. According to the data, the average molecular weight of the five polysaccharide fractions was between 4 816 and 993 935 Da. In all four types (CA, SC, PH1, and PH4), xylose was the most abundant monosaccharide, especially in PH1 and PH4. Both PH1 and PH4 were found to contain 1,4-β-d-Xylp as their main chain, as determined by nuclear magnetic resonance (NMR) spectroscopy. Additional research into CA and SC's antioxidant potential is required since they both showed potent in vitro antioxidant activities.

Keywords: Antioxidant activity; Bamboo shoot shells; Dendrocalamus brandisii; Polysaccharide; Structural characterization.