Perspective on the application of genome sequencing for monkeypox virus surveillance

Virol Sin. 2023 Apr;38(2):327-333. doi: 10.1016/j.virs.2023.03.006. Epub 2023 Mar 25.

¹ School of Public Health, Baotou Medical College, Baotou, 014040, China; NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 100052, China.
² NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 100052, China.
³ School of Public Health, Baotou Medical College, Baotou, 014040, China; NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 100052, China. Electronic address: tanwj@ivdc.chinacdc.cn.

Abstract

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Whole genome sequencing of MPXV is crucial for monitoring emerging variants and assessing their potential pathogenicity.
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The critical steps of mNGS, encompassing nucleic acid extraction, library preparation, sequencing, and data analysis, are concisely explained.
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Optimization strategies for sample pre-processing, virus enrichment, and sequencing platform selection are deliberated.
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Conducting next-generation sequencing and third-generation sequencing concurrently is highly recommended.