In-vitro assessment of a novel plant rhizobacterium, Citrobacter freundii, for degrading and biocontrol of food mycotoxin deoxynivalenol

Bilal Murtaza; Xiaoyu Li; Liming Dong; Muhammad Kashif Saleemi; Mazhar Iqbal; Saima Majeed; Ashiq Ali; Gen Li; Bowen Jin; Lili Wang; Yongping Xu

doi:10.1016/j.toxicon.2023.107095

In-vitro assessment of a novel plant rhizobacterium, Citrobacter freundii, for degrading and biocontrol of food mycotoxin deoxynivalenol

Toxicon. 2023 May:227:107095. doi: 10.1016/j.toxicon.2023.107095. Epub 2023 Mar 25.

Authors

Bilal Murtaza¹, Xiaoyu Li², Liming Dong³, Muhammad Kashif Saleemi⁴, Mazhar Iqbal⁵, Saima Majeed⁶, Ashiq Ali⁴, Gen Li⁷, Bowen Jin⁷, Lili Wang², Yongping Xu⁸

Affiliations

¹ School of Bioengineering, Dalian University of Technology, Dalian, 116024, China. Electronic address: bilalmurtaza50@gmail.com.
² School of Bioengineering, Dalian University of Technology, Dalian, 116024, China; Center for Food Safety of Animal Origin, Ministry of Education, Dalian University of Technology, Dalian, 116600, China.
³ Key Laboratory of Cleaner Production and Integrated Resource Utilization of China National Light Industry, Beijing Technology and Business University, Beijing, 100048, China.
⁴ Department of Pathology, University of Agriculture, Faisalabad, Pakistan.
⁵ National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan.
⁶ Institute of Biochemistry, Biotechnology and Bioinformatics (IBBB), The Islamia University, Bahawalpur, 63100, Pakistan.
⁷ School of Bioengineering, Dalian University of Technology, Dalian, 116024, China.
⁸ School of Bioengineering, Dalian University of Technology, Dalian, 116024, China; Center for Food Safety of Animal Origin, Ministry of Education, Dalian University of Technology, Dalian, 116600, China. Electronic address: xyping@dlut.edu.cn.

PMID: 36972839
DOI: 10.1016/j.toxicon.2023.107095

Abstract

Deoxynivalenol (DON) is one of the most harmful and well-known toxins present in food and animal feed throughout the world. Citrobacter freundii (C. freundii-ON077584), a novel DON-degrading strain, was isolated from rice root-linked soil samples. The degrading properties, including DON concentrations, incubation pH, incubation temperatures, bacterial concentrations, and acid treatment effect on degradation, were evaluated. At pH 7 and an incubation temperature of 37 °C, C. freundii demonstrated the capability to degrade more than 90% of DON. The degraded products of DON were identified as 3-keto-DON and DOM-1, which were confirmed by High Performance Liquid Chromatography (HPLC) and Ultra-Performance Liquid Chromatography hyphenated with Tandem Mass Spectrometry (UPLC-MS/MS) analyses. The mechanism of DON degradation into 3-keto-DON and DOM-1 by this bacterial strain will be further explored to identify and purify novel degrading enzymes that can be cloned to the microorganism and added to the animal feed to degrade the DON in the digestion tract.

Keywords: Bacteria; Biodegradation; Citrobacter freundii; Deoxynivalenol (DON); High performance liquid chromatography (HPLC).

MeSH terms

Animals
Bacteria / metabolism
Chromatography, Liquid
Citrobacter freundii / metabolism
Food Contamination / analysis
Food Contamination / prevention & control
Mycotoxins* / analysis
Tandem Mass Spectrometry

Substances

deoxynivalenol
deepoxy-deoxynivalenol
Mycotoxins
3-keto-deoxynivalenol