Evaluation of an in-house IgM/IgG lateral flow assay for serodiagnosis of human brucellosis

Rajeswari Shome; Sudipta Patra; Muneera Mohamed Sahib; G Shanmugam; Shiva Dubey; Somy Skariah; Samer Shamshad; Nagendra Nath Barman; Durlav Prasad Bora; Arijit Shome; Nagalingam Mohandoss; Bibek Ranjan Shome

doi:10.1016/j.ijmmb.2023.01.006

Evaluation of an in-house IgM/IgG lateral flow assay for serodiagnosis of human brucellosis

Indian J Med Microbiol. 2023 Mar-Apr:42:55-58. doi: 10.1016/j.ijmmb.2023.01.006. Epub 2023 Feb 3.

Affiliations

¹ Indian Council for Agricultural Research, National Institute of Veterinary Epidemiology and Disease Informatics, Bengalore, 560064, India. Electronic address: rajeswarishome@gmail.com.
² Indian Council for Agricultural Research, National Institute of Veterinary Epidemiology and Disease Informatics, Bengalore, 560064, India.
³ College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, 781022, India.

PMID: 36967218
DOI: 10.1016/j.ijmmb.2023.01.006

Abstract

This study aimed to evaluate the diagnostic efficacy of an in-house lateral flow assay (LFA) for the detection of IgM/IgG anti-Brucella antibodies for rapid serodiagnosis of human brucellosis. Three groups of sera samples including 476 from high-risk individuals, 27 from culture-confirmed patients, and 43 from healthy blood donors were used for evaluation of LFA. In comparison with iELISA, the sensitivity, specificity, and accuracy of LFA were >95%, >99%, and 99% respectively. Considering the very good agreement, accuracy, simplicity, and rapidity, LFAs might be useful as a point of care test for the diagnosis of human brucellosis in resource-limited laboratories.

Keywords: ELISA; Human brucellosis; Lateral flow assay (LFA); RBPT; SAT.

MeSH terms

Antibodies, Bacterial
Brucellosis* / diagnosis
Enzyme-Linked Immunosorbent Assay
Humans
Immunoglobulin G
Immunoglobulin M
Sensitivity and Specificity
Serologic Tests

Substances

Immunoglobulin M
Immunoglobulin G
Antibodies, Bacterial