Role of calcium sensor protein module CBL-CIPK in abiotic stress and light signaling responses in green algae

Abstract

Ca²⁺ signaling is an important biological process that enable to perceive and communicate information in the cell. Our current understanding of the signaling system suggests that plants and animals have certain differences in signal-sensing mechanisms. The Ca²⁺-mediated CBL-CIPK module has emerged as a major sensor responder network for Ca²⁺ signaling and has been speculated to be involved in plant terrestrial life adaptation. This module has previously been reported in Archaeplastids, Chromalveolates, and Excavates. In our experimental analysis of Chlamydomonas reinhardtii CBLs, we proved that the CrCBL1 protein interacts with Phototropin and Channelrhodopsin, and the expression of CrCBLs is modulated by light. Further analysis using chlorophyte and streptophyte algal sequences allowed us to identify the differences that have evolved in CBL and CIPK proteins since plants have progressed from aquatic to terrestrial habitats. Moreover, an investigation of Klebsormidium CBL and CIPK genes led us to know that they are abiotic stress stimuli-responsive, indicating that their role was defined very early during terrestrial adaptations. Structure-based prediction and Ca²⁺-binding assays indicated that the KnCBL1 protein in Klebsormidium showed a typical Ca²⁺-binding pocket. In summary, the results of this study suggest that these stress-responsive proteins enable crosstalk between Ca²⁺ and light signaling pathways very early during plant adaptation from aquatic to terrestrial habitats.

Keywords: Algae; CBL; Ca(2+); Channelrhodopsin; Chlamydomonas reinhardtii; Klebsormidium nitens; Phototropin.