Generation of a PPP1CA knockout human pluripotent stem cell line via CRISPR/Cas9

Xiaoman Wang; Jiaan Sun; Chong Fu; Chang Liu

doi:10.1016/j.scr.2023.103077

Generation of a PPP1CA knockout human pluripotent stem cell line via CRISPR/Cas9

Stem Cell Res. 2023 Jun:69:103077. doi: 10.1016/j.scr.2023.103077. Epub 2023 Mar 21.

Authors

Xiaoman Wang¹, Jiaan Sun¹, Chong Fu², Chang Liu³

Affiliations

¹ Department of Emergency, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450007, China.
² Henan Institute of Metrology, No. 10 Baifo Road, Guancheng District, Zhengzhou 450008, China.
³ Department of Emergency, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450007, China. Electronic address: liuchangzzch@126.com.

PMID: 36965407
DOI: 10.1016/j.scr.2023.103077

Abstract

The Protein phosphatase 1 catalytic subunit alpha (PPP1CA) is a alpha subunit of the PP1 complex, which known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Here, a PPP1CA knockout human embryonic stem cell line, WAe009-A-B, was generated using the CRISPR/Cas9 system to further study the function of PPP1CA. The cell line confirmed with pluripotency, normal karyotype and differentiation potential.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

CRISPR-Cas Systems* / genetics
Cell Line
Embryonic Stem Cells / metabolism
Human Embryonic Stem Cells* / metabolism
Humans
Protein Phosphatase 1 / genetics
Protein Phosphatase 1 / metabolism

Substances

Protein Phosphatase 1
PPP1CA protein, human