Generation of a TBX20-knockout human embryonic stem line by CRISPR/Cas9 system

Xiaodan Wu; Xiantao Wang

doi:10.1016/j.scr.2023.103082

Generation of a TBX20-knockout human embryonic stem line by CRISPR/Cas9 system

Stem Cell Res. 2023 Jun:69:103082. doi: 10.1016/j.scr.2023.103082. Epub 2023 Mar 21.

Authors

Xiaodan Wu¹, Xiantao Wang²

Affiliations

¹ Department of Cardiology, The First Affiliated Hospital of Guangxi Medical University & Guangxi Key Laboratory of Precision Medicine in Cardio-cerebrovascular Diseases Control and Prevention & Guangxi Clinical Research Center for Cardio-cerebrovascular Diseases, China.
² Department of Cardiology, The First Affiliated Hospital of Guangxi Medical University & Guangxi Key Laboratory of Precision Medicine in Cardio-cerebrovascular Diseases Control and Prevention & Guangxi Clinical Research Center for Cardio-cerebrovascular Diseases, China. Electronic address: wangxiantao@gxmu.edu.cn.

PMID: 36963213
DOI: 10.1016/j.scr.2023.103082

Abstract

The TBX20 gene plays a crucial role in embryonic development and has been involved in various diseases, such as heart defects, intellectual disability, and cancer. Herein, we have established a TBX20-knockout human embryonic stem cell line (WAe009-A-84) that maintains stem cell-like features, pluripotency, a normal karyotype, and the ability to differentiate into all three germ layers in vivo. This cell line will be a valuable resource for exploring TBX20's role in human development and could have significant implications for regenerative medicine and disease modeling.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

CRISPR-Cas Systems* / genetics
Cell Line
Embryonic Stem Cells / metabolism
Human Embryonic Stem Cells* / metabolism
Humans
T-Box Domain Proteins / genetics
T-Box Domain Proteins / metabolism

Substances

TBX20 protein, human
T-Box Domain Proteins