Rapid Biodegradation of the Organophosphorus Insecticide Acephate by a Novel Strain Burkholderia sp. A11 and Its Impact on the Structure of the Indigenous Microbial Community

Xiaozhen Wu; Wen-Juan Chen; Ziqiu Lin; Yaohua Huang; Talaat N-M El Sebai; Nasser Alansary; Dalia E El-Hefny; Sandhya Mishra; Pankaj Bhatt; Huixiong Lü; Shaohua Chen

doi:10.1021/acs.jafc.2c07861

Rapid Biodegradation of the Organophosphorus Insecticide Acephate by a Novel Strain Burkholderia sp. A11 and Its Impact on the Structure of the Indigenous Microbial Community

J Agric Food Chem. 2023 Apr 5;71(13):5261-5274. doi: 10.1021/acs.jafc.2c07861. Epub 2023 Mar 24.

Authors

Xiaozhen Wu^{1

2}, Wen-Juan Chen^{1

2}, Ziqiu Lin^{1

2}, Yaohua Huang^{1

2}, Talaat N-M El Sebai³, Nasser Alansary^{1

4}, Dalia E El-Hefny⁵, Sandhya Mishra⁶, Pankaj Bhatt⁷, Huixiong Lü^{1

8}, Shaohua Chen^{1

2}

Affiliations

¹ State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangdong Provincial Key Laboratory of Agricultural & Rural Pollution Abatement and Environmental Safety, Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou 510642, China.
² Guangdong Laboratory for Lingnan Modern Agriculture, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China.
³ Department of Agricultural Microbiology, Agricultural and Biology Research Institute, National Research Centre, El-Buhouth Street, 12622 Dokki, Cairo, Egypt.
⁴ Plant Protection Department, Division of Pesticides, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt.
⁵ Pesticide Residues and Environmental Pollution Department, Central of Agricultural Pesticide Laboratory, Agricultural Research Center, 12618 Dokki, Giza, Egypt.
⁶ Environmental Technologies Division, CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow 226001, India.
⁷ Department of Agricultural & Biological Engineering, Purdue University, West Lafayette, Indiana 47906, United States.
⁸ College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China.

PMID: 36962004
DOI: 10.1021/acs.jafc.2c07861

Abstract

The acephate-degrading microbes that are currently available are not optimal. In this study, Burkholderia sp. A11, an efficient degrader of acephate, presented an acephate-removal efficiency of 83.36% within 56 h (100 mg·L^-1). The A11 strain has a broad substrate tolerance and presents a good removal effect in the concentration range 10-1600 mg·L^-1. Six metabolites from the degradation of acephate were identified, among which the main products were methamidophos, acetamide, acetic acid, methanethiol, and dimethyl disulfide. The main degradation pathways involved include amide bond breaking and phosphate bond hydrolysis. Moreover, strain A11 successfully colonized and substantially accelerated acephate degradation in different soils, degrading over 90% of acephate (50-200 mg·kg^-1) within 120 h. 16S rDNA sequencing results further confirmed that the strain A11 gradually occupied a dominant position in the soil microbial communities, causing slight changes in the diversity and composition of the indigenous soil microbial community structure.

Keywords: Burkholderia sp; acephate; biodegradation; degradation behavior; pesticide residues.

MeSH terms

Biodegradation, Environmental
Burkholderia* / metabolism
Insecticides* / chemistry
Organophosphorus Compounds
Organothiophosphorus Compounds* / chemistry
Phosphoramides
Soil

Substances

acephate
Insecticides
Organophosphorus Compounds
Organothiophosphorus Compounds
Phosphoramides
Soil