Encapsulation of Nepeta hormozganica and Nepeta dschuprensis essential oils in shrimp chitosan NPs: Enhanced antifungal activity

Mina Amighi; Mahboobeh Zahedifar; Hamidreza Alizadeh; Maryam Payandeh

doi:10.1016/j.ijbiomac.2023.124112

Encapsulation of Nepeta hormozganica and Nepeta dschuprensis essential oils in shrimp chitosan NPs: Enhanced antifungal activity

Int J Biol Macromol. 2023 May 31:238:124112. doi: 10.1016/j.ijbiomac.2023.124112. Epub 2023 Mar 21.

Authors

Mina Amighi¹, Mahboobeh Zahedifar², Hamidreza Alizadeh³, Maryam Payandeh⁴

Affiliations

¹ Department of Plant Protection Faculty of Agriculture, University of Jiroft, Jiroft 7867161167, Iran.
² Department of Chemistry, Faculty of Science, University of Jiroft, Jiroft 7867161167, Iran. Electronic address: mzahedi@ujiroft.ac.ir.
³ Department of Plant Protection Faculty of Agriculture, University of Jiroft, Jiroft 7867161167, Iran. Electronic address: hamidalizadeh@ujiroft.ac.ir.
⁴ Department of Biology, Faculty of Science, University of Jiroft, Jiroft 7867161167, Iran.

PMID: 36948343
DOI: 10.1016/j.ijbiomac.2023.124112

Abstract

This study investigated the encapsulation of Nepeta hormozganica (NHEO) and Nepeta Dschuprensis (NDEO) essential oils into chitosan nanoparticles (CSN) via a simple ionic gelation method with tripolyphosphate (TPP). Chitosan (CS) is prepared by demineralizing, deproteinizing, and deacetylating shrimp shells waste in high yield (70.2 %). SEM, TEM, FT-IR, TGA, and XRD techniques were employed to characterize the encapsulated essential oils ((NHEO-CSN) and (NDEO-CSN)). The prepared EOs-CSN and CSN are found with particle sizes of 100-150 nm and 400-500 nm, respectively, and regular distribution. The encapsulation efficiency of encapsulated Nepeta hormozganica and Dschuprensis essential oils were found to be 73.64 % and 75.91 %, respectively. The synthesized nanocapsules were evaluated for antifungal activity against Fusarium oxysporium, Sclerotinia sclerotiorum, Pythium aphanidermatum, Alternaria alternata, Rhizactonia Solani, and Botrytis cinerea. Antifungal studies show that encapsulated essential oils increased antifungal efficiency by up to 100 %.

Keywords: Biofungicide; NDEO-CSN; NHEO-CSN.

MeSH terms

Antifungal Agents / pharmacology
Chitosan*
Nanoparticles*
Nepeta*
Oils, Volatile* / pharmacology
Particle Size
Spectroscopy, Fourier Transform Infrared

Substances

Oils, Volatile
Antifungal Agents
Chitosan