A multifunctional nanocomposite coated with a BSA membrane for cascaded nitric oxide therapy

Rong Ren; David H Bremner; Wenling Chen; Anhua Shi; Tong Wang; Ying Wang; Chengji Wang; Junzi Wu; Li-Min Zhu

doi:10.1016/j.ijbiomac.2023.124087

A multifunctional nanocomposite coated with a BSA membrane for cascaded nitric oxide therapy

Int J Biol Macromol. 2023 May 31:238:124087. doi: 10.1016/j.ijbiomac.2023.124087. Epub 2023 Mar 20.

Authors

Rong Ren¹, David H Bremner², Wenling Chen³, Anhua Shi⁴, Tong Wang¹, Ying Wang¹, Chengji Wang⁵, Junzi Wu⁶, Li-Min Zhu⁷

Affiliations

¹ College of Biological Science and Medical Engineering, Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, Donghua University, Shanghai 201620, China.
² School of Science, Engineering and Technology, Abertay University, Kydd Building, Dundee DD1 1HG, Scotland, UK.
³ School of Clinical Medicine, Yunnan University of Chinese Medicine, Kunming 650500, China.
⁴ The Key Laboratory of Microcosmic Syndrome Differentiation, Education Department of Yunnan, Yunnan University of Chinese Medicine, Kunming 650500, China.
⁵ Shanghai Laboratory Animal Research Center, Shanghai 201203, China.
⁶ The Key Laboratory of Microcosmic Syndrome Differentiation, Education Department of Yunnan, Yunnan University of Chinese Medicine, Kunming 650500, China. Electronic address: xnfz@ynutcm.edu.cn.
⁷ College of Biological Science and Medical Engineering, Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, Donghua University, Shanghai 201620, China. Electronic address: lzhu@dhu.edu.cn.

PMID: 36940766
DOI: 10.1016/j.ijbiomac.2023.124087

Abstract

Gas therapy based on nitric oxide (NO) has emerged as a potential therapeutic approach for cancer, and in conjunction with multi-mode combination therapy, offers new possibilities for achieving significant hyperadditive effects. In this study, an integrated AI-MPDA@BSA nanocomposite for diagnosis and treatment was constructed for PDA based photoacoustic imaging (PAI) and cascade NO release. Natural NO donor L-arginine (L-Arg) and photosensitizer (PS) IR780 were loaded into mesoporous polydopamine (MPDA). Bovine serum albumin (BSA) was conjugated to the MPDA to increase the dispersibility and biocompatibility of the nanoparticles, as well as to serve as a gatekeeper controlling IR780 release from the MPDA pores. The AI-MPDA@BSA produced singlet oxygen (¹O₂) and converted it into NO through a chain reaction based on L-Arg, enabling a combination of photodynamic therapy and gas therapy. Moreover, due to the photothermal properties of MPDA, the AI-MPDA@BSA performed good photothermal conversion, which allowed photoacoustic imaging. As expected, both in vitro and in vivo studies have confirmed that the AI-MPDA@BSA nanoplatform has a significant inhibitory effect on cancer cells and tumors, and no apparent systemic toxicity or side effects were detected during the treatment period.

Keywords: Gas therapy; Mesoporous polydopamine; Nitric oxide.

MeSH terms

Humans
Nanoparticles*
Neoplasms* / therapy
Nitric Oxide
Phototherapy / methods
Serum Albumin, Bovine

Substances

Serum Albumin, Bovine
Nitric Oxide
3-(2'-pyridyldithio)benzyldiazoacetate