Gaining access to acetyl-CoA by peroxisomal surface display

Thomas Perrot; Sébastien Besseau; Nicolas Papon; Vincent Courdavault

doi:10.1016/j.synbio.2023.02.003

Gaining access to acetyl-CoA by peroxisomal surface display

Synth Syst Biotechnol. 2023 Mar 6;8(2):224-226. doi: 10.1016/j.synbio.2023.02.003. eCollection 2023 Jun.

Authors

Thomas Perrot¹, Sébastien Besseau¹, Nicolas Papon², Vincent Courdavault¹

Affiliations

¹ Biomolécules et Biotechnologies Végétales, BBV, EA2106, Université de Tours, Tours, France.
² Univ Angers, Univ Brest, IRF, SFR ICAT, F-49000, Angers, France.

Abstract

Synthetic biology is constantly making progress for producing compounds on demand. Recently, Yocum and collaborators have developed an outstanding approach based on the anchoring of biosynthetic enzymes to the peroxisomal membrane. This allowed access to an untapped resource of acetyl-CoA and stimulated the synthesis of a valuable polyketide.

Keywords: 2-PS, 2-Pyrone synthase; Acc1, Acetyl-CoA carboxylase; Acetyl-CoA; Cat2, Carnitine acetyl-CoA transferase; CoA, Coenzyme A; Enzymes colocalization; Peroxisomal membrane; Polyketides; Synthetic biology; TAL, Triacetic acid lactone.