High-yield vesicle-packaged recombinant protein production from E. coli

Tara A Eastwood; Karen Baker; Bree R Streather; Nyasha Allen; Lin Wang; Stanley W Botchway; Ian R Brown; Jennifer R Hiscock; Christopher Lennon; Daniel P Mulvihill

doi:10.1016/j.crmeth.2023.100396

High-yield vesicle-packaged recombinant protein production from E. coli

Cell Rep Methods. 2023 Feb 2;3(2):100396. doi: 10.1016/j.crmeth.2023.100396. eCollection 2023 Feb 27.

Authors

Affiliations

¹ School of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, UK.
² School of Chemistry and Forensics, University of Kent, Canterbury, Kent CT2 7NJ, UK.
³ Central Laser Facility, Research Complex at Harwell, Science and Technology Facilities Council, Rutherford Appleton Laboratory, Harwell, Didcot, Oxford OX11 0QX, UK.
⁴ Fujifilm-Diosynth Biotechnologies UK, Ltd., Belasis Avenue, Billingham TS23 1LH, UK.

Abstract

We describe an innovative system that exports diverse recombinant proteins in membrane-bound vesicles from E. coli. These recombinant vesicles compartmentalize proteins within a micro-environment that enables production of otherwise challenging insoluble, toxic, or disulfide-bond containing proteins from bacteria. The release of vesicle-packaged proteins supports isolation from the culture and allows long-term storage of active protein. This technology results in high yields of vesicle-packaged, functional proteins for efficient downstream processing for a wide range of applications from discovery science to applied biotechnology and medicine.

Keywords: disulfide bond; extracellular vesicles; recombinant protein.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biotechnology / methods
Escherichia coli Proteins* / genetics
Escherichia coli* / genetics
Recombinant Proteins / genetics

Substances

Recombinant Proteins
Escherichia coli Proteins

Abstract

Publication types

MeSH terms

Substances

Grants and funding