Transcriptome and molecular regulatory mechanisms analysis of gills in the black tiger shrimp Penaeus monodon under chronic low-salinity stress

Yun-Dong Li; Meng-Ru Si; Shi-Gui Jiang; Qi-Bin Yang; Song Jiang; Li-Shi Yang; Jian-Hua Huang; Xu Chen; Fa-Lin Zhou; ErChao Li

doi:10.3389/fphys.2023.1118341

Transcriptome and molecular regulatory mechanisms analysis of gills in the black tiger shrimp Penaeus monodon under chronic low-salinity stress

Front Physiol. 2023 Mar 1:14:1118341. doi: 10.3389/fphys.2023.1118341. eCollection 2023.

Authors

Yun-Dong Li^{1

2

3

4}, Meng-Ru Si², Shi-Gui Jiang², Qi-Bin Yang³, Song Jiang², Li-Shi Yang², Jian-Hua Huang², Xu Chen³, Fa-Lin Zhou^{2

3

4}, ErChao Li^{1

4}

Affiliations

¹ Key Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province, Hainan Aquaculture Breeding Engineering Research Center, College of Marine Sciences, Hainan University, Haikou, China.
² Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China.
³ Key Laboratory of Efficient Utilization and Processing of Marine Fishery Resources of Hainan Province, Sanya Tropical Fisheries Research Institute, Sanya, China.
⁴ Hainan Yazhou Bay Seed Laboratory, Sanya, China.

Abstract

Background: Salinity is one of the main influencing factors in the culture environment and is extremely important for the survival, growth, development and reproduction of aquatic animals. Methods: In this study, a comparative transcriptome analysis (maintained for 45 days in three different salinities, 30 psu (HC group), 18 psu (MC group) and 3 psu (LC group)) was performed by high-throughput sequencing of economically cultured Penaeus monodon. P. monodon gill tissues from each treatment were collected for RNA-seq analysis to identify potential genes and pathways in response to low salinity stress. Results: A total of 64,475 unigenes were annotated in this study. There were 1,140 upregulated genes and 1,531 downregulated genes observed in the LC vs. HC group and 1,000 upregulated genes and 1,062 downregulated genes observed in the MC vs. HC group. In the LC vs. HC group, 583 DEGs significantly mapped to 37 signaling pathways, such as the NOD-like receptor signaling pathway, Toll-like receptor signaling pathway, and PI3K-Akt signaling pathway; in the MC vs. HC group, 444 DEGs significantly mapped to 28 signaling pathways, such as the MAPK signaling pathway, Hippo signaling pathway and calcium signaling pathway. These pathways were significantly associated mainly with signal transduction, immunity and metabolism. Conclusions: These results suggest that low salinity stress may affect regulatory mechanisms such as metabolism, immunity, and signal transduction in addition to osmolarity in P. monodon. The greater the difference in salinity, the more significant the difference in genes. This study provides some guidance for understanding the low-salt domestication culture of P. monodon.

Keywords: Chronic low-salinity stress; Penaeus Monodon; adaptation mechanisms; osmoregulation; transcriptome.

Grants and funding

This research was supported by National Key R & D Program of China (2022YFD2401900); China Agriculture Research System (CARS-48); Hainan Yazhou Bay Seed Laboratory (Project of B21Y10701, B21HJ0701); Central Public Interest Scientific Institution Basal Research Fund, South China Sea Fisheries Research Institute, CAFS (2020ZD01, 2021SD13); Hainan Provincial Natural Science Foundation of China (320QN359, 322RC806); Guangdong Basic and Applied Basic Research Foundation (2020A1515110200); Guangzhou Science and Technology Planning Project (202102020208); and Hainan Provincial Association for Science and Technology of Young Science and Technology Talents Innovation Plan Project (QCQTXM202206).