The applications of animal models in phage therapy: An update

Fazal Mehmood Khan; Prasanth Manohar; Vijay Singh Gondil; Nancy Mehra; Greater Kayode Oyejobi; Nelson Odiwuor; Tauseef Ahmad; Guangtao Huang

doi:10.1080/21645515.2023.2175519

The applications of animal models in phage therapy: An update

Hum Vaccin Immunother. 2023 Dec 31;19(1):2175519. doi: 10.1080/21645515.2023.2175519. Epub 2023 Mar 19.

Authors

Fazal Mehmood Khan^{1

2

3

4}, Prasanth Manohar⁵, Vijay Singh Gondil^{4

6}, Nancy Mehra⁷, Greater Kayode Oyejobi^{4

8

9}, Nelson Odiwuor^{4

10

11}, Tauseef Ahmad¹², Guangtao Huang^{13

14

15}

Affiliations

¹ College of Civil and Transportation Engineering, Shenzhen University, Shenzhen, China.
² Shenzhen Key Laboratory of Marine Microbiome Engineering, Institute for Advanced Study, Shenzhen University, Shenzhen, China.
³ Institute for Innovative Development of Food Industry, Shenzhen University, Shenzhen, China.
⁴ Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei, China.
⁵ School of Biosciences and Technology, Vellore Institute of Technology, Vellore, India.
⁶ Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA.
⁷ Department of Pediatrics, Advanced Pediatrics Centre, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
⁸ Department of Microbiology, Osun State University, Osogbo, Nigeria.
⁹ School of Pharmaceutical Sciences, Wuhan University, Wuhan, Hubei, China.
¹⁰ International College, University of Chinese Academy of Sciences, Beijing, China.
¹¹ Microbiology, Sino-Africa Joint Research Centre, Nairobi, Kenya.
¹² Department of Epidemiology and Health Statistics, School of Public Health, Southeast University, Nanjing, China.
¹³ Department of Burn and Plastic Surgery, Shenzhen Institute of Translational Medicine, The First Affiliated Hospital of Shenzhen University, Shenzhen, China.
¹⁴ Department of Burns and Plastic Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi, China.
¹⁵ Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Abstract

The rapid increase in antibiotic resistance presents a dire situation necessitating the need for alternative therapeutic agents. Among the current alternative therapies, phage therapy (PT) is promising. This review extensively summarizes preclinical PT approaches in various in-vivo models. PT has been evaluated in several recent clinical trials. However, there are still several unanswered concerns due to a lack of appropriate regulation and pharmacokinetic data regarding the application of phages in human therapeutic procedures. In this review, we also presented the current state of PT and considered how animal models can be used to adapt these therapies for humans. The development of realistic solutions to circumvent these constraints is critical for advancing this technology.

Keywords: ESKAPE Pathogens; Endolysin; Phage therapy; animal infection model; antimicrobial resistance.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Anti-Bacterial Agents / therapeutic use
Bacterial Infections* / drug therapy
Bacteriophages* / physiology
Drug Resistance, Multiple, Bacterial
Humans
Models, Animal
Phage Therapy* / methods

Substances

Anti-Bacterial Agents

Grants and funding

This work is supported by a Research Fund for International Young Scientists from the National Natural Science Foundation of China (NSFC) to Dr. Fazal Mehmood Khan of Grant number: 32250410292.This work is financially supported by the National Natural Science Foundation of China [81960353 and 82172238], the Science and Technology Support Project of Guizhou Province [2020-1Y332], Sanming Project of Medicine in Shenzhen[SZSM202111020], Science and Technology Cooperation Project of Zunyi Science and Technology Bureau [HZ-2019-50] and Health Commission Science and Technology Fund Project of Guizhou Province [gzwjkj2019-1-151].