Ultrasensitive amperometric determination of hand, foot and mouth disease based on gold nanoflower modified microelectrode

Anal Chim Acta. 2023 Apr 29:1252:341034. doi: 10.1016/j.aca.2023.341034. Epub 2023 Mar 2.

Abstract

Given the widespread use of point-of-care testing for diagnosis of disease, micro-scale electrochemical deoxyribonucleic acid (DNA) biosensors have become a promising area of research owing to its fast mass transfer, high current density and rapid response. In this study, a gold nanoparticles modified gold microelectrode (AuNPs/Au-Me) was constructed to determine the hand, foot and mouth disease (HFMD)-related gene. The noble metal nanoparticles modification yielded ca. 7.4-fold increase in electroactive surface area of microelectrode, and the signal for HFMD-related gene was largely magnified. Under optimal conditions, the biosensor exhibited salient selectivity and sensitivity with a low detection limit of 0.3 fM (S/N = 3), which is sufficient for clinical diagnosis of HFMD. Additionally, the developed AuNPs/Au-Me was successfully applied to determining the polymerase chain reaction (PCR) amplified products of target gene. Thus, the electrochemical DNA biosensor possesses great potential in early-stage diagnosis and long-term monitoring of various disease.

Keywords: Electrochemical DNA biosensor; Foot and mouth disease; Gold nanoparticles; Hand; Microelectrode.

MeSH terms

  • Biosensing Techniques*
  • DNA / genetics
  • Electrochemical Techniques
  • Gold
  • Hand, Foot and Mouth Disease* / diagnosis
  • Humans
  • Limit of Detection
  • Metal Nanoparticles*
  • Microelectrodes

Substances

  • Gold
  • DNA