Generation of liver metastases in a mouse model using ultrasound-guided intravenous injection

Amanda Labora; Hailey Lee; Charlotte Chan; Erin Tabornal; Thuc Le; Khalid Rashid; Evan Abt; Takanobu Yamao; Hanna Mandl; Amanda Creech; Alykhan Premji; Luyi Li; Jason Link; Nanping Wu; Caius Radu; Timothy Donahue

doi:10.1016/j.xpro.2023.102163

Generation of liver metastases in a mouse model using ultrasound-guided intravenous injection

STAR Protoc. 2023 Mar 15;4(2):102163. doi: 10.1016/j.xpro.2023.102163. Online ahead of print.

Authors

Affiliations

¹ Department of Surgery, University of California, Los Angeles, Los Angeles, CA 90095, USA. Electronic address: alabora@mednet.ucla.edu.
² Department of Molecular and Medical Pharmacology, Ahmanson Translational Imaging Division, University of California, Los Angeles, Los Angeles, CA 90095, USA.
³ Department of Surgery, University of California, Los Angeles, Los Angeles, CA 90095, USA.
⁴ Department of Surgery, University of California, Los Angeles, Los Angeles, CA 90095, USA. Electronic address: tdonahue@mednet.ucla.edu.

Abstract

Here, we present a protocol to generate a murine model of liver metastasis by directly injecting tumor cells into the portal vein under ultrasound guidance. We describe steps for animal and cell preparation and two techniques for injecting tumor cells. One technique is freehand, while the other technique is device-assisted using a 3D-printed prototype device. Finally, we describe tumor surveillance with bioluminescent imaging.

Keywords: Cancer; Cell Biology; Health Sciences; Model Organisms.

Abstract

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