Resveratrol alleviates high glucose-induced oxidative stress and apoptosis in rat cardiac microvascular endothelial cell through AMPK/Sirt1 activation

Jinyu Li; Zikai Feng; Binger Lu; Xinzhe Fang; Danmei Huang; Bin Wang

doi:10.1016/j.bbrep.2023.101444

Resveratrol alleviates high glucose-induced oxidative stress and apoptosis in rat cardiac microvascular endothelial cell through AMPK/Sirt1 activation

Biochem Biophys Rep. 2023 Mar 1:34:101444. doi: 10.1016/j.bbrep.2023.101444. eCollection 2023 Jul.

Authors

Jinyu Li¹, Zikai Feng², Binger Lu², Xinzhe Fang³, Danmei Huang³, Bin Wang³

Affiliations

¹ Institution of Drug Clinical Trial, The Second Affiliated Hospital, Shantou University Medical College, Shantou, 515041, China.
² Department of Clinical Pharmacy, Division of Pharmacy, The First Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong, China.
³ Department of Pharmacology, Shantou University Medical College, Shantou, 515041, China.

Abstract

Diabetic cardiomyopathy (DCM) is a common complication of diabetes. DCM causes extensive lesions on cardiac microvasculature that is predominantly cardiac microvascular endothelial cells (CMECs). Reducing high glucose (HG)-induced damage such as oxidative damage and apoptosis could alleviate the development of DCM. The natural polyphenol resveratrol (RSV) is widely suggested as a cardioprotective agent that protect against DCM. However, limited evidence supports the protection of RSV against oxidative damage and apoptosis and study on the direct effects of RSV in CMEC is missing. Therefore, the current paper aimed to illustrate if RSV could attenuate oxidative stress and apoptosis in CMEC and to investigate the underlying mechanisms. Our data showed that HG elevated reactive oxygen species, malondialdehyde, decreased superoxide dismutase activity, increased apoptotic cell percentage in CMEC, which were reversed by RSV administration. In addition, RSV demonstrated antioxidative and anti-apoptotic effects in CMEC through AMPK/Sirt1 activation, further confirmed by AMPK inhibition or Sirt1 silencing. This study provides new evidence to support RSV as a potential cardioprotective alternative in treating DCM.

Keywords: AMPK/Sirt1; Apoptosis; Cardiac microvascular endothelial cell; Diabetic cardiomyopathy; Oxidative stress; Resveratrol.