Hierarchical and urchin-like chitosan/hydroxyapatite microspheres as drug-laden cell carriers

Song Chen; Hao Li; Yajia Bai; Jianan Zhang; Toshiyuki Ikoma; Di Huang; Xiaona Li; Weiyi Chen

doi:10.1016/j.ijbiomac.2023.124039

Hierarchical and urchin-like chitosan/hydroxyapatite microspheres as drug-laden cell carriers

Int J Biol Macromol. 2023 May 31:238:124039. doi: 10.1016/j.ijbiomac.2023.124039. Epub 2023 Mar 14.

Authors

Song Chen¹, Hao Li², Yajia Bai², Jianan Zhang², Toshiyuki Ikoma³, Di Huang⁴, Xiaona Li², Weiyi Chen⁵

Affiliations

¹ College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan, 030024, China. Electronic address: chensong2009@126.com.
² College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan, 030024, China.
³ Department of Materials Science and Engineering, School of Materials and Chemical Technology, Tokyo Institute of Technology, Tokyo 152-8550, Japan.
⁴ College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan, 030024, China. Electronic address: huangjw2067@163.com.
⁵ College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan, 030024, China. Electronic address: chenweiyi@tyut.edu.cn.

PMID: 36921830
DOI: 10.1016/j.ijbiomac.2023.124039

Abstract

Biopolymer/hydroxyapatite (HAp) composites are one type of the most promising materials for a variety of biomedical applications. In this study, hierarchical and urchin-like chitosan/HAp nanowire (HU-CS/HAp NW) microspheres were for the first time synthesized by in situ hydrothermal treatment of chitosan/HAp (CS/HAp) microspheres in the acetic acid solution. The results indicate that HU-CS/HAp NW microspheres were spherical in morphology with a diameter of 100-300 μm. Their surface was mainly constructed by numerous HAp NWs with the diameter of 80-120 nm and showed a hierarchical and urchin-like nanofibrous architecture. It was found that the acidic hydrothermal treatment caused an in situ conversion of HAp NPs to HAp NWs. In vitro biocompatible evaluation indicates that HU-CS/HAp NW microspheres showed an enhanced cell attachment and proliferation due to the presence of hierarchical and urchin-like architecture. Furthermore, HU-CS/HAp NW microspheres showed a good adsorption capacity for tetracycline hydrochloride (model drug, one of the most representative antibiotics) with a higher adsorption capacity than CS/HAp microspheres and well maintained their antibacterial efficacy to inhibit the growth of bacteria: Escherichia coli and Staphylococcus aureus. Thus, the present HU-CS/HAp NW microspheres would be applicable as novel drug-laden cell carriers.

Keywords: Composite microspheres; Hierarchical and urchin-like nanofibrous architecture.

MeSH terms

Anti-Bacterial Agents / pharmacology
Chitosan* / pharmacology
Drug Carriers
Durapatite* / pharmacology
Microspheres

Substances

Durapatite
Chitosan
Drug Carriers
Anti-Bacterial Agents