Pseudotyped Viruses for Lyssavirus

Wenbo Wang; Caifeng Long; Lan Wang; Youchun Wang

doi:10.1007/978-981-99-0113-5_10

Pseudotyped Viruses for Lyssavirus

Adv Exp Med Biol. 2023:1407:191-208. doi: 10.1007/978-981-99-0113-5_10.

Authors

Wenbo Wang¹, Caifeng Long¹, Lan Wang², Youchun Wang^{3

4}

Affiliations

¹ Division of Monoclonal Antibody Products, National Institutes for Food and Drug Control (NIFDC) and WHO Collaborating Center for Standardization and Evaluation of Biologicals, Beijing, China.
² Division of Monoclonal Antibody Products, National Institutes for Food and Drug Control (NIFDC) and WHO Collaborating Center for Standardization and Evaluation of Biologicals, Beijing, China. wanglan@nifdc.org.cn.
³ Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. wangyc@nifdc.org.cn.
⁴ Institute of Medical Biology, Chinese Academy of Medicine Sciences & Peking Union Medical College, Kunming, China. wangyc@nifdc.org.cn.

PMID: 36920698
DOI: 10.1007/978-981-99-0113-5_10

Abstract

Lyssaviruses, which belong to the family Rhabdoviridae, are enveloped and bullet-shaped ssRNA viruses with genetic diversity. All members of Lyssavirus genus are known to infect warm-blooded animals and cause the fatal disease rabies. The rabies virus (RABV) in lyssavirus is the major pathogen to cause fatal rabies. The pseudotyped RABV is constructed to study the biological functions of G protein and evaluation of anti-RABV products including vaccine-induced antisera, rabies immunoglobulins (RIG), neutralizing mAbs, and other antiviral inhibitors. In this chapter, we focus on RABV as a representative and describe the construction of RABV G protein bearing pseudotyped virus and its applications. Other non-RABV lyssaviruses are also included.

Keywords: Glycoprotein; Lyssavirus; Pseudovirus; RABV.

MeSH terms

Animals
Lyssavirus* / genetics
Rabies Vaccines* / genetics
Rabies Vaccines* / metabolism
Rabies virus* / genetics
Rabies*
Rhabdoviridae Infections*
Viral Pseudotyping

Substances

Rabies Vaccines