Pseudotyped Viruses for Marburgvirus and Ebolavirus

Li Zhang; Shou Liu; Youchun Wang

doi:10.1007/978-981-99-0113-5_6

Pseudotyped Viruses for Marburgvirus and Ebolavirus

Adv Exp Med Biol. 2023:1407:105-132. doi: 10.1007/978-981-99-0113-5_6.

Authors

Li Zhang¹, Shou Liu¹, Youchun Wang^{2

3}

Affiliations

¹ Division of HIV/AIDS and Sex-transmitted Virus Vaccines, National Institutes for Food and Drug Control (NIFDC) and WHO Collaborating Center for Standardization and Evaluation of Biologicals, Beijing, China.
² Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. wangyc@nifdc.org.cn.
³ Institute of Medical Biology, Chinese Academy of Medicine Sciences & Peking Union Medical College, Kunming, China. wangyc@nifdc.org.cn.

PMID: 36920694
DOI: 10.1007/978-981-99-0113-5_6

Abstract

Marburg virus (MARV) and Ebola virus (EBOV) of the Filoviridae family are the most lethal viruses in terms of mortality rate. However, the development of antiviral treatment is hampered by the requirement for biosafety level-4 (BSL-4) containment. The establishment of BSL-2 pseudotyped viruses can provide important tools for the study of filoviruses. This chapter summarizes general information on the filoviruses and then focuses on the construction of replication-deficient pseudotyped MARV and EBOV (e.g., lentivirus system and vesicular stomatitis virus system). It also details the potential applications of the pseudotyped viruses, including neutralization antibody detection, the study of infection mechanisms, the evaluation of antibody-dependent enhancement, virus entry inhibitor screening, and glycoprotein mutation analysis.

Keywords: Drug screening; EBOV; Lentivirus; MARV; Mutation analysis; VSV neutralization.

MeSH terms

Antiviral Agents / pharmacology
Ebolavirus* / genetics
Glycoproteins
Hemorrhagic Fever, Ebola* / prevention & control
Humans
Marburgvirus* / genetics
Viral Pseudotyping

Substances

Antiviral Agents
Glycoproteins