Isoelectric point barcode and similarity analysis with the earth mover's distance for identification of species origin of raw meat

Tian Liu; Youli Tian; Yiren Cao; Zihao Wang; Genhan Zha; Weiwen Liu; Li Wei; Hua Xiao; Qiang Zhang; Chengxi Cao

doi:10.1016/j.foodres.2023.112600

Isoelectric point barcode and similarity analysis with the earth mover's distance for identification of species origin of raw meat

Food Res Int. 2023 Apr:166:112600. doi: 10.1016/j.foodres.2023.112600. Epub 2023 Feb 18.

Authors

Tian Liu¹, Youli Tian², Yiren Cao³, Zihao Wang¹, Genhan Zha², Weiwen Liu¹, Li Wei⁴, Hua Xiao⁵, Qiang Zhang⁶, Chengxi Cao⁷

Affiliations

¹ School of Sensing Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
² School of Sensing Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China; School of Life Sciences and Biotechnology, State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, China.
³ School of Sensing Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China; School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
⁴ Shanghai 6(th) People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, China.
⁵ School of Life Sciences and Biotechnology, State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: huaxiao@sjtu.edu.cn.
⁶ School of Sensing Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China; School of Life Sciences and Biotechnology, State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: billy_z@sjtu.edu.cn.
⁷ School of Life Sciences and Biotechnology, State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, China; Shanghai 6(th) People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, China. Electronic address: cxcao@sjtu.edu.cn.

PMID: 36914325
DOI: 10.1016/j.foodres.2023.112600

Abstract

In this work, by combining the microcolumn isoelectric focusing (mIEF) and similarity analysis with the earth mover's distance (EMD) metric, we proposed the concept of isoelectric point (pI) barcode for the identification of species origin of raw meat. At first, we used the mIEF to analyze 14 meat species, including 8 species of livestock and 6 species of poultry, to generate 140 electropherograms of myoglobin/hemoglobin (Mb/Hb) markers. Secondly, we binarized the electropherograms and converted them into the pI barcodes that only showed the major Mb/Hb bands for the EMD analysis. Thirdly, we efficiently developed the barcode database of 14 meat species and successfully used the EMD method to identify 9 meat products thanks to the high throughput of mIEF and the simplified format of the barcode for similarity analysis. The developed method had the merits of facility, rapidity and low cost. The developed concept and method had evident potential to the facile identification of meat species.

Keywords: Barcode; Hemoglobin (Hb); Isoelectric focusing; Isoelectric point; Meat identification; Myoglobin (Mb).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Algorithms*
Hemoglobins*
Isoelectric Point
Meat / analysis

Substances

Hemoglobins