Improved production of bacterial cellulose by Komagataeibacter europaeus employing fruit extract as carbon source

Yi Sheng Tseng; Anil Kumar Patel; Chiu-Wen Chen; Cheng-Di Dong; Reeta Rani Singhania

doi:10.1007/s13197-022-05451-y

Improved production of bacterial cellulose by Komagataeibacter europaeus employing fruit extract as carbon source

J Food Sci Technol. 2023 Mar;60(3):1054-1064. doi: 10.1007/s13197-022-05451-y. Epub 2022 Jul 20.

Authors

Yi Sheng Tseng^{1

2}, Anil Kumar Patel^{2

3}, Chiu-Wen Chen^{1

2

3}, Cheng-Di Dong^{1

2

3}, Reeta Rani Singhania^{2

3}

Affiliations

¹ Institute of Aquatic Science and Technology, National Kaohsiung University of Science and Technology, Kaohsiung City, 81157 Taiwan.
² Department of Marine Environmental Engineering, National Kaohsiung University of Science and Technology, Kaohsiung City, 81157 Taiwan.
³ Sustainable Environment Research Center, National Kaohsiung University of Science and Technology, Kaohsiung City, 81157 Taiwan, Republic of China.

Abstract

Bacterial cellulose (BC) has attracted worldwide attention owing to its tremendous properties and versatile applications. BC has huge market demand, however; its production is still limited hence important to explore the economically and technically feasible bioprocess for its improved production. The current study is based on improving the bioprocess for BC production employing Komagataeibacter europeaus 14148. Physico-chemical parameters have been optimized e.g., initial pH, incubation temperature, incubation period, inoculum size, and carbon source for maximum BC production. The study employed crude and/or a defined carbon source in the production medium. Hestrin and Schramm (HS) medium was used for BC production with initial pH 5.5 at 30 °C after 7 days of incubation under static conditions. The yield of BC obtained from fruit juice extracted from orange, papaya, mango and banana were higher than other sugars employed. The maximum BC yield of 3.48 ± 0.16 g/L was obtained with papaya extract having 40 g/L reducing sugar concentration and 3.47 ± 0.05 g/L BC was obtained with orange extract having 40 g/L reducing sugar equivalent in the medium. BC yield was about three-fold higher than standard HS medium. Fruit extracts can be employed as sustainable and economic substrates for BC production to replace glucose and fructose.

Supplementary information: The online version contains supplementary material available at 10.1007/s13197-022-05451-y.

Keywords: Bacterial cellulose; Bioprocess; Biorefinery; Carbon source; Komagataeibacter.