Halonatronomonas betaini gen. nov., sp. nov., a haloalkaliphilic isolate from soda lake capable of betaine degradation and proposal of Halarsenatibacteraceae fam. nov. and Halothermotrichaceae fam. nov. within the order Halanaerobiales

Yulia V Boltyanskaya; Vadim V Kevbrin; Denis S Grouzdev; Ekaterina N Detkova; Veronika V Koziaeva; Andrey A Novikov; Tatjana N Zhilina

doi:10.1016/j.syapm.2023.126407

Halonatronomonas betaini gen. nov., sp. nov., a haloalkaliphilic isolate from soda lake capable of betaine degradation and proposal of Halarsenatibacteraceae fam. nov. and Halothermotrichaceae fam. nov. within the order Halanaerobiales

Syst Appl Microbiol. 2023 May;46(3):126407. doi: 10.1016/j.syapm.2023.126407. Epub 2023 Feb 26.

Authors

Yulia V Boltyanskaya¹, Vadim V Kevbrin², Denis S Grouzdev³, Ekaterina N Detkova¹, Veronika V Koziaeva⁴, Andrey A Novikov⁵, Tatjana N Zhilina¹

Affiliations

¹ Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, 33, bld. 2, Leninsky Ave., Moscow 119071, Russia.
² Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, 33, bld. 2, Leninsky Ave., Moscow 119071, Russia. Electronic address: kevbrin@inmi.ru.
³ SciBear OU, Tartu mnt 67/1-13b, Tallinn 10115, Estonia.
⁴ Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, 33, bld. 2, Leninsky Ave., Moscow 119071, Russia.
⁵ Gubkin University, Leninsky Ave., 65/1, Moscow 119991, Russia.

PMID: 36906934
DOI: 10.1016/j.syapm.2023.126407

Abstract

A search for the organisms responsible for anaerobic betaine degradation in soda lakes resulted in isolation of a novel bacterial strain, designated Z-7014^T. The cells were Gram-stain-negative, non-endospore-forming rods. Growth occurred at 8-52 °C (optimum 40-45 °C), pH 7.1-10.1 (optimum pH 8.1-8.8) and 1.0-3.5 M Na⁺ (optimum 1.8 M), i.e. it can be regarded as a haloalkaliphile. The strain utilized a limited range of substrates, mostly peptonaceous but not amino acids, and was able to degrade betaine. Growth on betaine occurred only in the presence of peptonaceous substances which could not be replaced by vitamins. The G + C content of the genomic DNA of strain Z-7014^T was 36.1 mol%. The major cellular fatty acids (>5% of the total) were C_16:0 DMA, C_{18: 0} DMA, C_16:1ω8, C_16:0, C_18:1 DMA, C_16:1 DMA, C_18:1ω9, and C_18:0. Phylogenetic analysis of the 16S rRNA gene sequence revealed that strain Z-7014^T formed a distinct evolutionary lineage in the order Halanaerobiales with the highest similarity to Halarsenitibacter silvermanii SLAS-1^T (83.6%), Halothermothrix orenii H168^T (85.6%), and Halocella cellulosilytica DSM 7362^T (85.6%). AAI and POCP values between strain Z-7014^T and type strains of the order Halanaerobiales were 51.7-57.8%, and 33.8-58.3%, respectively. Based on polyphasic results including phylogenomic data, the novel strain could be distinguished from other genera, which suggests that strain Z-7014^T represents a novel species of a new genus, for which the name Halonatronomonas betaini gen. nov., sp. nov. is proposed. The type strain is Z-7014^T (=KCTC 25237^T = VKM B-3506^T). On the basis of phylogenomic data, it is also proposed to evolve two novel families Halarsenitibacteraceae fam. nov. and Halothermotrichaceae fam. nov. within the current order Halanaerobiales.

Keywords: Betaine degradation; Halarsenitibacteraceae fam. nov.; Haloalkaliphile; Halonatronomonas betaini gen. nov. sp. nov.; Halothermotrichaceae fam. nov.

MeSH terms

Bacteria* / chemistry
Bacteria* / cytology
Bacteria* / isolation & purification
Bacteria* / metabolism
Betaine / metabolism
Fatty Acids / analysis
Lakes* / microbiology
Phylogeny

Substances

Betaine
Fatty Acids