Identification of multifunctionality of grass carp (Ctenopharyngodon idella) TBK1 during bacterial infection

Hongyan Yu; Xuewen Jia; Yifan Pang; Huiqin Niu; Biao Du; Xiaoyan Xu; Jiale Li

doi:10.1016/j.fsi.2023.108630

Identification of multifunctionality of grass carp (Ctenopharyngodon idella) TBK1 during bacterial infection

Fish Shellfish Immunol. 2023 May:136:108630. doi: 10.1016/j.fsi.2023.108630. Epub 2023 Mar 10.

Authors

Hongyan Yu¹, Xuewen Jia¹, Yifan Pang¹, Huiqin Niu¹, Biao Du¹, Xiaoyan Xu², Jiale Li³

Affiliations

¹ Key Laboratory of Freshwater Aquatic Genetic Resources Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, China; Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai, China.
² Key Laboratory of Freshwater Aquatic Genetic Resources Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, China; Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China. Electronic address: xyxu@shou.edu.cn.
³ Key Laboratory of Freshwater Aquatic Genetic Resources Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, China; Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China. Electronic address: jlli@shou.edu.cn.

PMID: 36906050
DOI: 10.1016/j.fsi.2023.108630

Abstract

TBK1 is an atypical IκB kinase family member with a set of functions. It is involved in congenital immunization and autophagy in mammals. In this study, we reported that grass carp TBK1 gene expression could be upregulated by bacterial infection. Overexpression of TBK1 could decrease the number of adhesive bacteria in CIK cells. TBK1 could promote cellular migration, proliferation, vitality, and anti-apoptosis ability. Furthermore, the expression of TBK1 could activate the NF-κB signaling pathway by inducing inflammatory cytokines. In addition, we found that the grass carp TBK1 could cause the autophagy level of CIK cells within the decreasing level of p62 protein. Our finding indicated that TBK1 participated in grass carp innate immune progress and autophagy. This study provides evidence of the positive regulation of TBK1 in teleost innate immunity with its multiple functions. It thus may provide important information about the defense and immune mechanisms used by teleost against pathogens.

Keywords: Autophagy; Grass carp; Innate immunity; TBK1.

MeSH terms

Animals
Bacterial Infections* / veterinary
Carps* / genetics
Carps* / metabolism
Cytokines
Fish Diseases*
Fish Proteins
Immunity, Innate / genetics
Mammals / metabolism
Signal Transduction / genetics

Substances

Cytokines
Fish Proteins