The excessive use of pyrophosphate (PPi) anions as additives poses a serious threat to human health and the environment. Considering the current status of PPi probes, the development of metal-free auxiliary PPi probes has important applications. In this study, a novel near-infrared nitrogen and sulfur co-doped carbon dots (N,S-CDs) were prepared. The average particle size of N,S-CDs was 2.25 ± 0.32 nm with average height was 3.05 nm. The probe N,S-CDs showed a special response to PPi, and a good linear relationship was obtained with PPi concentrations ranging from 0 to 1 μM, with the limit of detection being 0.22 nM. Tap water and milk were used for practical inspection, and ideal experimental results were acquired. In addition, the probe N,S-CDs also showed good results in biological systems, such as cell and zebrafish experiments.
Keywords: Carbon dots; HeLa cells; Metal-free auxiliary; Pyrophosphate anions; Zebrafish.
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