Meyerozyma guilliermondii promoted the deposition of GSH type lignin by activating the biosynthesis and polymerization of monolignols at the wounds of potato tubers

Xiaoyuan Zheng; Xuejiao Zhang; Jinmei Zhao; William Oyom; Haitao Long; Ruirui Yang; Lumei Pu; Yang Bi; Dov Prusky

doi:10.1016/j.foodchem.2023.135688

Meyerozyma guilliermondii promoted the deposition of GSH type lignin by activating the biosynthesis and polymerization of monolignols at the wounds of potato tubers

Food Chem. 2023 Aug 1:416:135688. doi: 10.1016/j.foodchem.2023.135688. Epub 2023 Feb 17.

Authors

Xiaoyuan Zheng¹, Xuejiao Zhang¹, Jinmei Zhao¹, William Oyom¹, Haitao Long², Ruirui Yang¹, Lumei Pu², Yang Bi³, Dov Prusky⁴

Affiliations

¹ College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China.
² College of Science, Gansu Agricultural University, Lanzhou 730070, China.
³ College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China. Electronic address: biyang@gsau.edu.cn.
⁴ College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China; Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Rishon LeZion 7505101, Israel.

PMID: 36905709
DOI: 10.1016/j.foodchem.2023.135688

Abstract

Lignin is a crucial component in the wound tissue of tubers. The biocontrol yeast Meyerozyma guilliermondii increased the activities of phenylalanine ammonia lyase, cinnamate-4-hydroxylase, 4-coenzyme coenzyme A ligase, and cinnamyl alcohol dehydrogenase, and elevated the levels of coniferyl, sinapyl, and p-coumaryl alcohol. The yeast also enhanced the activities of peroxidase and laccase, as well as the content of hydrogen peroxide. The lignin promoted by the yeast was identified as guaiacyl-syringyl-p-hydroxyphenyl type using Fourier transform infrared spectroscopy and two-dimensional heteronuclear single quantum coherence nuclear magnetic resonance. Furthermore, a larger signal area for G₂, G₅, G'₆, S_{2, 6}, and S'_{2, 6} units was observed in the treated tubers, and the G'₂ and G₆ units were only detected in the treated tuber. Taken together, M. guilliermondii could promote deposition of guaiacyl-syringyl-p-hydroxyphenyl type lignin by activating the biosynthesis and polymerization of monolignols at the wounds of potato tubers.

Keywords: GSH; Healing; Lignin; Meyerozyma guilliermondii; Phenylpropanoid metabolism; Potato tuber.

MeSH terms

Lignin* / chemistry
Polymerization
Solanum tuberosum*

Substances

Lignin

Supplementary concepts

Meyerozyma guilliermondii