Isolation and Identification of Dendritic Cell Subsets from Human and Mouse Tumors

Abstract

Dendritic cells (DCs) are professional antigen-presenting cells (APCs) that have the ability to orchestrate adaptive and innate immune responses by antigen phagocytosis and T cell activation across different inflammatory settings such as tumor development. As specific DC identity and how these cells interact with their neighbors is still not fully understood, it remains a challenge to unravel DC heterogeneity, particularly in human cancers. In this chapter, we describe a protocol to isolate and characterize tumor-infiltrating DCs.

Keywords: DC identification; DC isolation; DC subsets; Dendritic cells; Human; Mouse; Tumors.