This study aimed to examine the sexual dimorphism effect of two-generation exposure to a high-fat diet (HFD) on the craniofacial growth of rat offspring. Ten eleven-week-old pregnant Wistar rats were fed either a control or HFD from day 7 of pregnancy until the end of lactation. Twelve male and female offspring from the control-diet-fed mothers were assigned to the CM (control male, n = 6) and CF (control female, n = 6) groups. The other twelve from the HFD-fed mothers were assigned to the HFD male (HFDM, n = 6) and HFD female (HFDF, n = 6) groups. HFDM and HFDF rats continued with an HFD. The offspring's weight and fasting blood sugar levels were measured every two weeks. The craniofacial and dental morphologies were studied from lateral X-rays of the head at ten weeks old. The HFDM rats showed an increased body weight and larger neurocranial parameters compared with the CM group. Furthermore, there were slightly significant differences in body weight and viscerocranial parameters between the rats in the HFDF and CF groups. In conclusion, two-generational exposure to an HFD had a greater effect on the male offspring's body weight and craniofacial morphology.
Keywords: cephalometric analysis; craniofacial growth; high-fat diet; lactation; pregnancy.