Effects of Schisandra chinensis Polysaccharide-Conjugated Selenium Nanoparticles on Intestinal Injury in Mice

Hongxu Du; Xiaoyan Tan; Zhangxun Li; Hong Dong; Lijuan Su; Zhengke He; Qi Ma; Shiqi Dong; Mythili Ramachandran; Juan Liu; Liting Cao

doi:10.3390/ani13050930

Effects of Schisandra chinensis Polysaccharide-Conjugated Selenium Nanoparticles on Intestinal Injury in Mice

Animals (Basel). 2023 Mar 4;13(5):930. doi: 10.3390/ani13050930.

Authors

Hongxu Du¹, Xiaoyan Tan¹, Zhangxun Li¹, Hong Dong², Lijuan Su¹, Zhengke He¹, Qi Ma^{1

3

4}, Shiqi Dong^{1

3

4}, Mythili Ramachandran⁵, Juan Liu^{1

3

4}, Liting Cao^{1

3

4}

Affiliations

¹ Department of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Southwest University, Chongqing 402460, China.
² Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, Beijing University of Agriculture, Beijing 102206, China.
³ National Center of Technology Innovation for Pigs (NCTIP-XD/C17), Chongqing 402460, China.
⁴ Immunology Research Center, Medical Research Institute, Southwest University, Chongqing 402460, China.
⁵ Department of Biochemistry and Molecular Medicine, University of California Davis, Sacramento, CA 95817, USA.

Abstract

Schisandra chinensis polysaccharide (SCP) is an experimental therapeutic for the treatment of intestinal injury. Selenium nanoparticle modification can improve the bioactivity of polysaccharides. In this study, SCP was firstly extracted and purified by a DEAE-52 column, then SCP-Selenium nanoparticles (SCP-Se NPs) were prepared, and the procedure was optimized. Thereafter, the obtained SCP-Se NPs were characterized by transmission electron microscope, X-ray diffraction, energy-dispersive X-ray spectroscopy, and Fourier transform infrared spectroscopy. The influence of different storage environments on the stability of colloidal SCP-Se NPs was also investigated. Finally, the therapeutic effects of SCP-Se NPs on LPS-induced intestinal inflammatory injuries in mice were evaluated. Results showed that the optimized SCP-Se NPs were amorphous, uniform, spherical particles with a diameter of 121 nm, and the colloidal solution was stable at 4 °C for at least 14 d. Moreover, SCP-Se NPs could more effectively alleviate LPS-induced diarrhea, intestinal tissue injury, and tight junction destruction and decrease the elevated expression levels of TNF-α, IL-1β, and IL-6 compared with SCP. These results demonstrate that SCP-Se NPs may alleviate LPS-induced enteritis through their anti-inflammatory effects, indicating that SCP-Se NPs can serve as a good candidate for preventing and treating enteritis in the livestock and poultry industry.

Keywords: Schisandra chinensis polysaccharide; intestinal inflammatory injury; selenium nanoparticle.

Grants and funding

This work was supported by Natural Science Foundation of Chongqing, China (CSTB2022NSCQ-MSX0470), the Open Project Program of Beijing Key Laboratory of Traditional Chinese Veterinary Medicine at Beijing University of Agriculture (BUAPSP202205), Fundamental Research Funds for the Central Universities (SWU120002, SWU-KT22012), and the Natural Science Foundation of Chongqing (cstc2021jcyj-msxmX0982).