Magnetospirillum sulfuroxidans sp. nov., capable of sulfur-dependent lithoautotrophy and a taxonomic reevaluation of the order Rhodospirillales

Veronika V Koziaeva; Dimitry Y Sorokin; Tatiana V Kolganova; Denis S Grouzdev

doi:10.1016/j.syapm.2023.126406

Magnetospirillum sulfuroxidans sp. nov., capable of sulfur-dependent lithoautotrophy and a taxonomic reevaluation of the order Rhodospirillales

Syst Appl Microbiol. 2023 May;46(3):126406. doi: 10.1016/j.syapm.2023.126406. Epub 2023 Feb 26.

Authors

Veronika V Koziaeva¹, Dimitry Y Sorokin², Tatiana V Kolganova³, Denis S Grouzdev⁴

Affiliations

¹ Skryabin Institute of Bioengineering, Research Centre of Biotechnology, Russian Academy of Sciences, Moscow, Russia. Electronic address: vkoziaieva@mail.ru.
² Winogradsky Institute of Microbiology, Research Centre of Biotechnology, Russian Academy of Sciences, Moscow, Russia; Department of Biotechnology, Delft University of Technology, Delft, The Netherlands.
³ Skryabin Institute of Bioengineering, Research Centre of Biotechnology, Russian Academy of Sciences, Moscow, Russia.
⁴ SciBear OU, Tallinn, Estonia.

PMID: 36898262
DOI: 10.1016/j.syapm.2023.126406

Abstract

A spiral-shaped, highly motile bacterium was isolated from freshwater sulfidic sediment. Strain J10^T is a facultative autotroph utilizing sulfide, thiosulfate, and sulfur as the electron donors in microoxic conditions. Despite high 16S rRNA gene sequence sequence identity to Magnetospirillum gryphiswaldense MSR-1 ^T (99.6 %), digital DNA-DNA hybridisation homology and average nucleotide identity between the two strains was of the different species level (25 % and 83 %, respectively). Strain J10^T is not magnetotactic. The DNA G + C content of strain J10^T is 61.9 %. The predominant phospholipid ester-linked fatty acids are C18:1ω7, C16:1ω7, and C16:0. Strain J10^T (=DSM 23205 ^T = VKM B-3486 ^T) is the first strain of the genus Magnetospirillum showing lithoautotrophic growth and is proposed here as a novel species, Magnetospirillum sulfuroxidans sp. nov. In addition, we propose to establish a framework for distinguishing genera and families within the order Rhodospirillales based on phylogenomic analysis using the threshold values for average amino acid identity at ̴ 72 % for genera and ̴ 60 % for families. According to this, we propose to divide the existing genus Magnetospirillum into three genera: Magnetospirillum, Paramagnetospirillum, and Phaeospirillum, constituting a separate family Magnetospirillaceae fam. nov. in the order Rhodospirillales. Furthermore, phylogenomic data suggest that this order should accomodate six more new family level groups including Magnetospiraceae fam. nov., Magnetovibrionaceae fam. nov., Dongiaceae fam. nov., Niveispirillaceae fam. nov., Fodinicurvataceae fam. nov., and Oceanibaculaceae fam. nov.

Keywords: Magnetospirillaceae; Magnetospirillum; Magnetotactic bacteria; Paramagnetospirillum; Rhodospirillaceae; Rhodospirillales.

MeSH terms

Bacterial Typing Techniques
DNA, Bacterial / genetics
Fatty Acids / chemistry
Magnetospirillum* / genetics
Phospholipids / chemistry
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA

Substances

RNA, Ribosomal, 16S
DNA, Bacterial
Fatty Acids
Phospholipids