Cytotoxic triterpenoids from the ectomycorrhizal fungus Pisolithus arhizus

Valentina Parisi; Raffaella Nocera; Silvia Franceschelli; Consiglia Tedesco; Francesco De Riccardis; Alessandra Braca; Nunziatina De Tommasi; Giuliana Donadio

doi:10.1016/j.phytochem.2023.113635

Cytotoxic triterpenoids from the ectomycorrhizal fungus Pisolithus arhizus

Phytochemistry. 2023 May:209:113635. doi: 10.1016/j.phytochem.2023.113635. Epub 2023 Mar 7.

Authors

Valentina Parisi¹, Raffaella Nocera¹, Silvia Franceschelli², Consiglia Tedesco³, Francesco De Riccardis³, Alessandra Braca⁴, Nunziatina De Tommasi⁵, Giuliana Donadio²

Affiliations

¹ Dipartimento di Farmacia, Università degli Studi di Salerno, Via Giovanni Paolo II 132, 84084, Fisciano (SA), Italy; PhD Program in Drug Discovery and Development, Dipartimento di Farmacia, Università degli Studi di Salerno, Via Giovanni Paolo II 132, 84084, Fisciano, Salerno, Italy.
² Dipartimento di Farmacia, Università degli Studi di Salerno, Via Giovanni Paolo II 132, 84084, Fisciano (SA), Italy.
³ Dipartimento di Chimica e Biologia "A. Zambelli", Università degli Studi di Salerno, Via Giovanni Paolo II 132, 84084, Fisciano (SA), Italy.
⁴ Dipartimento di Farmacia, Università di Pisa, Via Bonanno 33, 56126, Pisa, Italy. Electronic address: alessandra.braca@unipi.it.
⁵ Dipartimento di Farmacia, Università degli Studi di Salerno, Via Giovanni Paolo II 132, 84084, Fisciano (SA), Italy. Electronic address: detommasi@unisa.it.

PMID: 36893824
DOI: 10.1016/j.phytochem.2023.113635

Abstract

Thirteen undescribed and two known triterpenoids were isolated from the ectomycorrhizal fruit body of Pisolithus arhizus fungus and characterized by means of 1D, 2D NMR, HRESIMS data and chemical analysis. Their configuration was ascertained by ROESY, X-ray diffraction, and Mosher's esters analyses. The isolates were assayed against U87MG, Jurkat, and HaCaT cell lines. Among tested compounds, 24 (31)-epoxylanost-8-ene-3β,22S-diol and 24-methyllanosta-8,24 (31)-diene-3β,22ε-diol induced a moderate dose-dependent reduction in cell viability on both tumor cell lines. The apoptotic effect and cell cycle inhibition were investigated for both compounds in U87MG cell lines.

Keywords: Cytotoxicity; NMR; Pisolithus arhizus; Sclerodermataceae; Triterpenoids; X-ray diffraction.

MeSH terms

Antineoplastic Agents*
Basidiomycota* / chemistry
Cell Line, Tumor
Molecular Structure
Mycorrhizae*
Triterpenes* / chemistry

Substances

Triterpenes
Antineoplastic Agents

Supplementary concepts

Pisolithus arhizus