Lactiplantibacillus pentosus P2020 protects the hyperuricemia and renal inflammation in mice

Zhihuan Wang; Liqiong Song; Xianping Li; Yuchun Xiao; Yuanming Huang; Yue Zhang; Jintong Li; Mingding Li; Zhihong Ren

doi:10.3389/fnut.2023.1094483

Lactiplantibacillus pentosus P2020 protects the hyperuricemia and renal inflammation in mice

Front Nutr. 2023 Feb 20:10:1094483. doi: 10.3389/fnut.2023.1094483. eCollection 2023.

Authors

Zhihuan Wang¹, Liqiong Song¹, Xianping Li¹, Yuchun Xiao¹, Yuanming Huang¹, Yue Zhang², Jintong Li³, Mingding Li⁴, Zhihong Ren¹

Affiliations

¹ State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Research Units of Discovery of Unknown Bacteria and Function (2018 RU010), Chinese Academy of Medical Sciences, Beijing, China.
² Third Affiliated Hospital, Beijing University of Chinese Medicine, Beijing, China.
³ Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China.
⁴ Maiyata Institute for Beneficial Bacteria, Shaoxing, Zhejiang, China.

Abstract

Introduction: Hyperuricemia (HUA) is a common metabolic disease, and its prevalence has been increasing worldwide. Pharmaceutical drugs have been used for controlling HUA but they all have certain side effects, which thus calls for discovering alternative options including using treatment of probiotics to prevent the development of HUA.

Methods: We established HUA mice model induced by potassium oxonate and adenine and performed in vivo experiments to verify the ability to lower serum uric acid of Lactiplantibacillus pentosus P2020 (LPP), a probiotics stain extracted from Chinese pickle. We also tried to discussed the underlying mechanisms.

Results: Oral administration with LPP significantly decreased serum uric acid and reduced renal inflammatory response by downregulating multiple inflammation pathways including NK-kB, MAPK, and TNFα. We also found that LPP administration significantly promoted uric acid excretion by regulating expression of transporters in the kidney and ileum. In addition, LPP intake improved intestinal barrier function and modulated the composition of gut microbiota.

Discussion: These results suggest that probiotics LPP may have a promising potential to protect against development of HUA and HUA-related renal damage, and its working mechanisms involve regulation of inflammation pathways and expression of transporters in the kidney and ileum.

Keywords: Lactiplantibacillus pentosus; NF-kB; gut microbiota; hyperuricemia; intestine barrier function; kidney inflammation; probiotics; urate reabsorption transporter.